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The effect of low-level laser therapy on osteoclast differentiation: Clinical implications for tooth movement and bone density.
- Source :
-
Journal of dental sciences [J Dent Sci] 2024 Jul; Vol. 19 (3), pp. 1452-1460. Date of Electronic Publication: 2024 Mar 30. - Publication Year :
- 2024
-
Abstract
- Background/purpose: Osteoclast differentiation is crucial for orchestrating both tooth movement and the maintenance of bone density. Therefore, the current study sought to explore the impact of low-level laser therapy (LLLT) on osteoclast differentiation, functional gene expression, molecular signaling pathways, and orthodontic tooth movement in clinical settings.<br />Materials and Methods: The RAW 264.7 cell line served as the precursor for osteoclasts, and these cells underwent irradiation using a 808-nm LLLT. Osteoclast differentiation was assessed through tartrate-resistant acid phosphatase (TRAP) staining. Functional gene expression levels were evaluated using real-time quantitative polymerase chain reaction (RT-qPCR) while signaling molecules were examined through Western blot analysis. In the clinical study, 12 participants were enrolled. Their tooth movement was monitored using a TRIOS desktop scanner. Bone density measurements were conducted using Mimics software, which processed cone-beam computed tomography (CBCT) images exported in Digital Imaging and Communications in Medicine (DICOM) format.<br />Results: We found that LLLT effectively promoted receptor activator of nuclear factor-κB ligand (RANKL)-dependent osteoclast differentiation and the expression of osteoclast functional genes, including matrix metallopeptidase 9 (MMP9), nuclear factor of activated T-cells cytoplasmic 1(NFATc1), tartrate-resistant acid phosphatase (TRAP) and cathepsin K (CTSK) in RAW264.7 cells. Clinically, the cumulative tooth movement over 90 days was significantly higher in the laser group than in the control group.<br />Conclusion: Our research demonstrates that LLLT not only significantly promotes osteoclast differentiation but is also a valuable adjunct in orthodontic therapy.<br />Competing Interests: The authors have no conflicts of interest relevant to this article.<br /> (© 2024 Association for Dental Sciences of the Republic of China. Publishing services by Elsevier B.V.)
Details
- Language :
- English
- ISSN :
- 2213-8862
- Volume :
- 19
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of dental sciences
- Publication Type :
- Academic Journal
- Accession number :
- 39035342
- Full Text :
- https://doi.org/10.1016/j.jds.2024.03.023