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High-resolution mapping of linear epitopes from LiNTPDase2: Advancing leishmaniasis detection using optimized protein and peptide antigens.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2024 Oct; Vol. 110 (2), pp. 116448. Date of Electronic Publication: 2024 Jul 18. - Publication Year :
- 2024
-
Abstract
- Visceral Leishmaniasis, caused by Leishmania infantum, is a tropical neglected disease and the most dangerous form of Leishmaniasis. It occurs zoonotically, with domestic transmission posing risks to humans as dogs have high susceptibility and are natural reservoirs of the parasite. Given their epidemiological role, improvements are needed in diagnosing Canine Visceral Leishmaniasis (CVL). Thus, we mapped linear epitopes from the rLiNTPDase2 antigen through peptide microarray and identified six positive epitopes. Validation through peptide ELISA revealed three promising peptides with accuracies of 78.6%, 85.92%, and 79.59%. Their combination yielded 97.58% accuracy. Negative epitopes were also found, which interacted with CVL-negative and Chagas Disease positive samples. Their removal from the rLiNTPDase2 sequence resulted in the rNT2.neg, which obtained enhanced specificity over rLiNTPDase2. The rNT2.neg validation achieved 87.50% sensitivity, 90.55% specificity, and 93.5% accuracy within 127 CVL-positive and 96 CVL-negative samples. Therefore, three peptides and rNT2.neg show significant promise for CVL diagnosis.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Dogs
Sensitivity and Specificity
Epitopes immunology
Peptides immunology
Peptides chemistry
Enzyme-Linked Immunosorbent Assay methods
Protozoan Proteins immunology
Antigens, Protozoan immunology
Leishmania infantum immunology
Epitope Mapping
Dog Diseases diagnosis
Dog Diseases parasitology
Leishmaniasis, Visceral diagnosis
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 110
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 39029390
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2024.116448