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Seasonal dynamics of intestinal microbiota in juvenile Chinese mitten crab ( Eriocheir sinensis ) in the Yangtze Estuary.
- Source :
-
Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 Jul 04; Vol. 14, pp. 1436547. Date of Electronic Publication: 2024 Jul 04 (Print Publication: 2024). - Publication Year :
- 2024
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Abstract
- Introduction: In this study, the seasonal differences in the intestinal microbiota of Chinese mitten crab ( Eriocheir sinensis ) larvae were investigated at different sites in the intertidal zone of the Yangtze River Estuary.<br />Methods: 16S rRNA high-throughput sequencing technology was used to compare and analyze the microbial community structure in the intestines of juvenile crab from different seasons.<br />Results: The results showed that the main microbial phyla in all seasons and sites were Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria, which accounted for 97.1% of the total microbiota. Composition analysis revealed that the relative abundance of Proteobacteria decreased from summer to winter at each station, whereas Bacteroidetes showed the opposite trend. Alpha diversity analysis showed that species richness increased from summer to winter at the upstream site ( P < 0.05), but decreased at the downstream site ( P < 0.05), with no significant differences observed in other comparisons. Biomarker species analysis showed that juvenile crab exhibited a more specialized microbial community in summer compared with autumn and winter. Co-occurrence network analysis revealed that microbial interaction network complexity was lower in autumn compared with summer and autumn. Functional prediction analysis showed that the microbial community only exhibited seasonal differences in amino acid biosynthesis, cofactor, prosthetic group, electron carrier, and vitamin biosynthesis, aromatic compound degradation, nucleotide and nucleoside degradation, and tricarboxylic acid cycle pathways.<br />Discussion: The results indicated that the microbiota did not significantly differ among sites, and seasonal variation was a main factor influencing the differences in intestinal microbiota of Chinese mitten juvenile crab. Moreover, the microbial community was more complex in summer compared with autumn and winter.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2024 Qin, Wang, Wu, Sun and Zhao.)
- Subjects :
- Animals
China
High-Throughput Nucleotide Sequencing
Bacteria classification
Bacteria genetics
Bacteria isolation & purification
Phylogeny
Biodiversity
Larva microbiology
Bacteroidetes classification
Bacteroidetes genetics
Bacteroidetes isolation & purification
Proteobacteria genetics
Proteobacteria classification
Proteobacteria isolation & purification
Firmicutes genetics
Firmicutes classification
Firmicutes isolation & purification
DNA, Bacterial genetics
Rivers microbiology
Gastrointestinal Microbiome
Brachyura microbiology
Seasons
RNA, Ribosomal, 16S genetics
Estuaries
Subjects
Details
- Language :
- English
- ISSN :
- 2235-2988
- Volume :
- 14
- Database :
- MEDLINE
- Journal :
- Frontiers in cellular and infection microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 39027136
- Full Text :
- https://doi.org/10.3389/fcimb.2024.1436547