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Sex-related differences in hypertrophy response and cardiac expression of G protein-coupled estrogen receptor in rats with pressure overload.
- Source :
-
Gene [Gene] 2024 Nov 30; Vol. 928, pp. 148769. Date of Electronic Publication: 2024 Jul 20. - Publication Year :
- 2024
-
Abstract
- There is increasing evidence that gender impacts the onset and progression of cardiovascular pathology. However, it is vastly unclear how this variable determines the ultimate outcomes, particularly in the setting of pressure overload-induced left ventricular hypertrophy (LVH). This study was carried out to fill this gap, at least in part, by assessing myocardial expression of G protein-coupled estrogen receptor (GPER) in female and male rats afflicted with LVH. Both female and male rats underwent abdominal aorta banding to induce LVH or were kept intact as control groups. At the end of the experiment, carotid artery catheterization was performed to measure systolic (SBP) and diastolic (DBP) blood pressure. Fibrosis and cardiomyocyte cross-sectional area were assessed by conventional histological analyses. Protein and mRNA expression were evaluated by Western blot/immunofluorescence staining and real-time RT-PCR technique, respectively. In LVH groups, male rats exhibited higher SBP and DBP, heart weight to body weight ratio, and fibrosis compared with female rats. However, both sexes showed a similar increase in cardiomyocyte size after LVH induction. In female, but not in male rats, LVH instigated the GPER mRNA and protein expression in the heart. These results, confirm a significant interaction between gender and myocardial remodeling in terms of GPER expression. Thus, it can be argued that sex differences in the cardiac GPER expression may be responsible for sex differences in the pressure overload-induced LVH. In other words, the female heart seems to unleash stronger protection against pressure overload than that of males in light of a higher GPER expression.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024. Published by Elsevier B.V.)
- Subjects :
- Animals
Male
Female
Rats
Blood Pressure
Myocardium metabolism
Myocardium pathology
Sex Characteristics
Myocytes, Cardiac metabolism
Myocytes, Cardiac pathology
Fibrosis
Rats, Sprague-Dawley
Sex Factors
Receptors, G-Protein-Coupled metabolism
Receptors, G-Protein-Coupled genetics
Hypertrophy, Left Ventricular metabolism
Hypertrophy, Left Ventricular genetics
Hypertrophy, Left Ventricular pathology
Hypertrophy, Left Ventricular etiology
Receptors, Estrogen metabolism
Receptors, Estrogen genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0038
- Volume :
- 928
- Database :
- MEDLINE
- Journal :
- Gene
- Publication Type :
- Academic Journal
- Accession number :
- 39025340
- Full Text :
- https://doi.org/10.1016/j.gene.2024.148769