Green-synthesized copper oxide nanoparticles induce apoptosis and up-regulate HOTAIR and HOTTIP in pancreatic cancer cells.

Authors :: Hosseini Z
Ahmadi A
Shadi A
Hosseini SJ
Nikmanesh H
Source :: Nanomedicine (London, England) [Nanomedicine (Lond)] 2024; Vol. 19 (18-20), pp. 1629-1641. Date of Electronic Publication: 2024 Jul 16.
Publication Year :: 2024
Abstract: Aim: Cu <subscript>2</subscript> O nanoparticles were synthesized using an extract from S. latifolium algae (SLCu <subscript>2</subscript> O NPs). Their effect on PANC-1 cells and the expression of two drug resistance-related lncRNAs were evaluated in comparison with Arsenic trioxide. Materials & methods: SLCu <subscript>2</subscript> O NPs were characterized using XRD, SEM, and TEM microscopies. The effects of SLCu <subscript>2</subscript> O NPs on cell cytotoxicity, cell cycle, and apoptosis, and expression of two drug resistance-related lncRNAs were examined using MTT assay, flow cytometry, and real-time PCR, respectively. Results: SLCu <subscript>2</subscript> O NPs demonstrated anti-cancer properties against PANC-1 cells comparable to Arsenic trioxide, and the expression of lncRNAs increased upon treatment with them. Conclusion: SLCu <subscript>2</subscript> O NPs demonstrate anti-cancer properties against PANC-1 cells; however, using gene silencing strategies along with SLCu <subscript>2</subscript> O NPs is suggested.

Subjects :: Humans
Cell Line, Tumor
Antineoplastic Agents pharmacology
Antineoplastic Agents chemistry
Up-Regulation drug effects
Metal Nanoparticles chemistry
Gene Expression Regulation, Neoplastic drug effects
Arsenic Trioxide pharmacology
Arsenic Trioxide chemistry
Cell Proliferation drug effects
Copper chemistry
Copper pharmacology
Apoptosis drug effects
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
Pancreatic Neoplasms drug therapy
Pancreatic Neoplasms pathology
Pancreatic Neoplasms metabolism
Green Chemistry Technology

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