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Transmission of global clones of NDM-producing Enterobacterales and interspecies spread of IncX3 plasmid harbouring bla NDM-5 in Tokyo.

Authors :
Harada S
Aoki K
Nomura Y
Ohama Y
Araoka H
Hayama B
Sakurai T
Ueda A
Ishii Y
Tsutsumi T
Source :
Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2024 Sep; Vol. 38, pp. 309-316. Date of Electronic Publication: 2024 Jul 14.
Publication Year :
2024

Abstract

Objective: The aim of this study is to characterise the molecular characteristics of NDM-producing Enterobacterales, which have been on the increase in recent years in Japan, where IMP-producing bacteria are dominant among carbapenemase-producing Enterobacterales.<br />Methods: We collected 21 strains of NDM-producing Enterobacterales detected between 2015 and 2022 at five hospitals in Tokyo and performed illumina whole genome sequencing. For the seven selected strains, nanopore long-read sequencing was also performed to characterise the plasmids harbouring bla <subscript>NDM</subscript> .<br />Results: Fourteen strains were Escherichia coli and all carried bla <subscript>NDM-5</subscript> . Among these strains, eight and three were sequence type (ST) 410 and ST167, respectively, and both groups of strains were spread clonally in different hospitals. Two strains of Klebsiella pneumoniae ST147 carrying bla <subscript>NDM-1</subscript> were detected in a hospital, and these strains had also spread clonally. The remainder included Enterobacter hormaechei, Klebsiella quasipneumoniae, Citrobacter amalonaticus, and Klebsiella michiganensis. Plasmid analysis revealed that an identical IncX3 plasmid harbouring bla <subscript>NDM-5</subscript> was shared among four strains of different bacterial species (E. coli, C. amalonaticus, K. michiganensis, and E. hormaechei) detected at the same hospital. In addition, a Klebsiella quasipneumoniae strain detected at a different hospital also carried an IncX3 plasmid with a similar genetic structure.<br />Conclusions: Nosocomial spread of multiple multidrug-resistant global clones and transmission of IncX3 plasmids harbouring bla <subscript>NDM-5</subscript> among multiple species were detected as the major pathways of spread of NDM-producing Enterobacterales in Tokyo. Early detection of carriers and measures to prevent nosocomial spread are important to prevent further spread of NDM-producing organisms.<br /> (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Details

Language :
English
ISSN :
2213-7173
Volume :
38
Database :
MEDLINE
Journal :
Journal of global antimicrobial resistance
Publication Type :
Academic Journal
Accession number :
39004343
Full Text :
https://doi.org/10.1016/j.jgar.2024.06.020