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Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection.
- Source :
-
Nutrients [Nutrients] 2024 Jun 27; Vol. 16 (13). Date of Electronic Publication: 2024 Jun 27. - Publication Year :
- 2024
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Abstract
- Helicobacter pylori infection affects over 50% of the world's population and leads to chronic inflammation and gastric disorders, being the main pathogen correlated to gastric cancer development. Increasing antibiotic resistance levels are a major global concern and alternative treatments are needed. Soybean peptides and other compounds might be an alternative in the treatment to avoid, eradicate and/or control symptoms of H. pylori infection. This study aimed to characterize a lunasin-enriched soybean extract (LSE) using proteomics tools and to evaluate its antioxidant, anti-inflammatory and antibacterial properties against H. pylori infection. By LC-MS/MS analysis, 124 proteins were identified, with 2S albumin (lunasin and large-chain subunits) being the fourth most abundant protein (8.9%). Lunasin consists of 44 amino acid residues and an intramolecular disulfide bond. LSE at a low dose (0.0625 mg/mL) reduced ROS production in both H. pylori -infected and non-infected AGS gastric cells. This led to a significant reduction of 6.71% in the levels of pro-inflammatory interleukin (IL)-8. LSE also showed antibacterial activity against H. pylori , which can be attributed to other soybean proteins and phenolic compounds. Our findings suggest that LSE might be a promising alternative in the management of H. pylori infection and its associated symptoms.<br />Competing Interests: The authors declare no conflicts of interest.
- Subjects :
- Humans
Antioxidants pharmacology
Anti-Inflammatory Agents pharmacology
Tandem Mass Spectrometry
Reactive Oxygen Species metabolism
Cell Line, Tumor
Seed Storage Proteins pharmacology
Helicobacter pylori drug effects
Helicobacter Infections drug therapy
Glycine max chemistry
Proteomics methods
Plant Extracts pharmacology
Plant Extracts chemistry
Soybean Proteins pharmacology
Anti-Bacterial Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 2072-6643
- Volume :
- 16
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Nutrients
- Publication Type :
- Academic Journal
- Accession number :
- 38999803
- Full Text :
- https://doi.org/10.3390/nu16132056