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Hepatoprotective effects of resveratrol on α-amanitin-induced liver toxicity in rats.
- Source :
-
Toxicon : official journal of the International Society on Toxinology [Toxicon] 2024 Aug 28; Vol. 247, pp. 107855. Date of Electronic Publication: 2024 Jul 10. - Publication Year :
- 2024
-
Abstract
- Objective: The hepatoprotective effects of resveratrol against α-Amanitin (α-AMA)-induced liver toxicity were investigated in an experimental rat model, focusing on oxidative stress, inflammation, apoptosis, and liver function.<br />Methods: Thirty-two male Sprague-Dawley rats were divided into four groups (n = 8 per group): Control, resveratrol, α-AMA, and resveratrol+α-AMA. The resveratrol group received 20 mg/kg resveratrol orally for 7 days. The α-AMA group received 3 mg/kg α-AMA intraperitoneally on the 8th day. The resveratrol+α-AMA group received 20 mg/kg resveratrol orally (7 days) followed by 3 mg/kg α-AMA intraperitoneally on the 8th day. Liver tissues and blood samples were collected 48 h after α-amanitin administration for histopathological, immunohistochemical (NFkB, LC3B), and biochemical analyses (GSH, MDA, CAT, GPx, MPO, NOS, AST, ALT).<br />Results: α-AMA significantly increased AST and ALT levels, oxidative stress marker (MDA), and inflammatory marker (MPO), while reducing antioxidant levels (GSH, CAT, GPx) and NOS concentration (P < 0.001 for all parameters). Histopathological analysis showed severe liver damage with increased NFkB and LC3B expression. resveratrol treatment significantly reduced AST and ALT levels (P < 0.01 for both parameters), decreased MDA and MPO levels, and increased NOS concentration, GSH, CAT, and GPx levels (P < 0.05 for all parameters). Reduced NFkB and LC3B expression in the resveratrol+α-AMA group and showed histopathological improvements.<br />Conclusion: Resveratrol demonstrated substantial hepatoprotective effects against α-AMA induced liver toxicity by reducing oxidative stress, inflammation, and apoptosis, and improving liver function. These findings suggest that resveratrol could be a potential therapeutic agent for treating liver damage caused by potent hepatotoxins like α-AMA.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Male
Rats
Protective Agents pharmacology
Apoptosis drug effects
Stilbenes pharmacology
Resveratrol pharmacology
Alpha-Amanitin toxicity
Rats, Sprague-Dawley
Oxidative Stress drug effects
Liver drug effects
Liver pathology
Liver metabolism
Chemical and Drug Induced Liver Injury prevention & control
Chemical and Drug Induced Liver Injury drug therapy
Antioxidants pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3150
- Volume :
- 247
- Database :
- MEDLINE
- Journal :
- Toxicon : official journal of the International Society on Toxinology
- Publication Type :
- Academic Journal
- Accession number :
- 38996975
- Full Text :
- https://doi.org/10.1016/j.toxicon.2024.107855