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Large-pore covalent organic framework as solid phase extraction absorbentforefficientdetermination of polypeptide antibiotics in animal-derived foods.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 2024 Aug 16; Vol. 1730, pp. 465150. Date of Electronic Publication: 2024 Jul 08. - Publication Year :
- 2024
-
Abstract
- The precise determination of polypeptide antibiotics (PPTs) in foods has been always challenging because of the interference of various endogenous peptides in complex matrix. Herin, a novel large-pore covalent organic framework (TABPT-SPDA-COF) with accessible pore size of 7.9 nm was synthesized as a solid phase extraction (SPE) absorbent for efficiently enriching four PPTs existed in foods originating from animals. The parameters of SPE process were systematically optimized. Subsequently, four PPTs were determined by UHPLC-MS/MS. Under the optimal conditions, TABPT-SPDA-COF shows outstanding enrichment capacity for PPTs in contrast to commercial absorbents ascribed to size selectivity and multiple interaction effects. The method exhibits excellent linear range (0.005-100 ng mL <superscript>-1</superscript> ), satisfactory limits of detection (0.1 pg mL <superscript>-1</superscript> ) as well as relative recoveries (86.2-116 %). This work offers a practicable platform to monitor trace PPTs from complex animal-derived foodstuffs.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Chromatography, High Pressure Liquid methods
Food Contamination analysis
Solid Phase Extraction methods
Anti-Bacterial Agents analysis
Anti-Bacterial Agents isolation & purification
Anti-Bacterial Agents chemistry
Metal-Organic Frameworks chemistry
Tandem Mass Spectrometry methods
Limit of Detection
Peptides analysis
Peptides isolation & purification
Peptides chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3778
- Volume :
- 1730
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 38991603
- Full Text :
- https://doi.org/10.1016/j.chroma.2024.465150