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A synthetic TLR4 agonist significantly increases humoral immune responses and the protective ability of an MDCK-cell-derived inactivated H7N9 vaccine in mice.
- Source :
-
Archives of virology [Arch Virol] 2024 Jul 11; Vol. 169 (8), pp. 163. Date of Electronic Publication: 2024 Jul 11. - Publication Year :
- 2024
-
Abstract
- Antigenically divergent H7N9 viruses pose a potential threat to public health, with the poor immunogenicity of candidate H7N9 vaccines demonstrated in clinical trials underscoring the urgent need for more-effective H7N9 vaccines. In the present study, mice were immunized with various doses of a suspended-MDCK-cell-derived inactivated H7N9 vaccine, which was based on a low-pathogenic H7N9 virus, to assess cross-reactive immunity and cross-protection against antigenically divergent H7N9 viruses. We found that the CRX-527 adjuvant, a synthetic TLR4 agonist, significantly enhanced the humoral immune responses of the suspended-MDCK-cell-derived H7N9 vaccine, with significant antigen-sparing and immune-enhancing effects, including robust virus-specific IgG, hemagglutination-inhibiting (HI), neuraminidase-inhibiting (NI), and virus-neutralizing (VN) antibody responses, which are crucial for protection against influenza virus infection. Moreover, the CRX-527-adjuvanted H7N9 vaccine also elicited cross-protective immunity and cross-protection against a highly pathogenic H7N9 virus with a single vaccination. Notably, NI and VN antibodies might play an important role in cross-protection against lethal influenza virus infections. This study showed that a synthetic TLR4 agonist adjuvant has a potent immunopotentiating effect, which might be considered worth further development as a means of increasing vaccine effectiveness.<br /> (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
- Subjects :
- Animals
Mice
Dogs
Madin Darby Canine Kidney Cells
Female
Antibodies, Neutralizing immunology
Cross Protection immunology
Adjuvants, Immunologic administration & dosage
Adjuvants, Immunologic pharmacology
Adjuvants, Vaccine
Immunoglobulin G immunology
Immunoglobulin G blood
Influenza A Virus, H7N9 Subtype immunology
Toll-Like Receptor 4 agonists
Toll-Like Receptor 4 immunology
Influenza Vaccines immunology
Influenza Vaccines administration & dosage
Antibodies, Viral immunology
Immunity, Humoral
Vaccines, Inactivated immunology
Orthomyxoviridae Infections prevention & control
Orthomyxoviridae Infections immunology
Mice, Inbred BALB C
Subjects
Details
- Language :
- English
- ISSN :
- 1432-8798
- Volume :
- 169
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Archives of virology
- Publication Type :
- Academic Journal
- Accession number :
- 38990396
- Full Text :
- https://doi.org/10.1007/s00705-024-06082-8