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BTB and CNC homology 1 deficiency disrupts intestinal IgA secretion through regulation of polymeric immunoglobulin receptor expression.
- Source :
-
American journal of physiology. Gastrointestinal and liver physiology [Am J Physiol Gastrointest Liver Physiol] 2024 Sep 01; Vol. 327 (3), pp. G414-G423. Date of Electronic Publication: 2024 Jul 09. - Publication Year :
- 2024
-
Abstract
- Immunoglobulin A (IgA)-mediated mucosal immunity is important for the host because it contributes to reducing infection risk and to establishing host-microbe symbiosis. BTB and CNC homology 1 (Bach1) is a transcriptional repressor with physiological and pathophysiological functions that are of particular interest for their relation to gastrointestinal diseases. However, Bach1 effects on IgA-mediated mucosal immunity remain unknown. For this study using Bach1-deficient ( Bach1 <superscript>-/-</superscript> ) mice, we investigated the function of Bach1 in IgA-mediated mucosal immunity. Intestinal mucosa, feces, and plasma IgA were examined using immunosorbent assay. After cell suspensions were prepared from Peyer's patches and colonic lamina propria, they were examined using flow cytometry. The expression level of polymeric immunoglobulin receptor (pIgR), which plays an important role in the transepithelial transport of IgA, was evaluated using Western blotting, quantitative real-time PCR, and immunohistochemistry. Although no changes in the proportions of IgA-producing cells were observed, the amounts of IgA in the intestinal mucosa were increased in Bach1 <superscript>-/-</superscript> mice. Furthermore, plasma IgA was increased in Bach1 <superscript>-/-</superscript> mice, but fecal IgA was decreased, indicating that Bach1 <superscript>-/-</superscript> mice have abnormal secretion of IgA into the intestinal lumen. In fact, Bach1 deficiency reduced pIgR expression in colonic mucosa at both the protein and mRNA levels. In the human intestinal epithelial cell line LS174T, suppression of Bach1 reduced pIgR mRNA stability. In contrast, the overexpression of Bach1 increased pIgR mRNA stability. These results demonstrate that Bach1 deficiency causes abnormal secretion of IgA into the intestinal lumen via suppression of pIgR expression. NEW & NOTEWORTHY The transcriptional repressor Bach1 has been implicated in diverse intestinal functions, but the effects of Bach1 on IgA-mediated mucosal immunity remain unclear. We demonstrate here that Bach1 deficiency causes abnormal secretion of IgA into the intestinal lumen, although the proportions of IgA-producing cells were not altered. Furthermore, Bach1 regulates the expression of pIgR, which plays an important role in the transepithelial transport of IgA, at the posttranscriptional level.
- Subjects :
- Animals
Mice
Humans
Immunoglobulin A metabolism
Immunity, Mucosal
Mice, Inbred C57BL
Immunoglobulin A, Secretory metabolism
Peyer's Patches metabolism
Peyer's Patches immunology
Gene Expression Regulation
Basic-Leucine Zipper Transcription Factors genetics
Basic-Leucine Zipper Transcription Factors metabolism
Basic-Leucine Zipper Transcription Factors deficiency
Receptors, Polymeric Immunoglobulin genetics
Receptors, Polymeric Immunoglobulin metabolism
Intestinal Mucosa metabolism
Intestinal Mucosa immunology
Mice, Knockout
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1547
- Volume :
- 327
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Gastrointestinal and liver physiology
- Publication Type :
- Academic Journal
- Accession number :
- 38981617
- Full Text :
- https://doi.org/10.1152/ajpgi.00215.2023