Back to Search
Start Over
Bombyx mori UFL1 facilitates BmNPV proliferation by regulating host cell apoptosis through PERK.
- Source :
-
Archives of insect biochemistry and physiology [Arch Insect Biochem Physiol] 2024 Jul; Vol. 116 (3), pp. e22127. - Publication Year :
- 2024
-
Abstract
- Ubiquitin-fold modifier 1 (UFM1) is attached to protein substrates through the sequential activity of an E1 (UBA5)-E2 (UFC1)-E3 (UFL1) cascade. UFL1 is the E3 ligase for UFMylation in vertebrates. However, there have been no studies on UFL1 in silkworm to date. In this study, we identified a UFL1 ortholog in Bombyx mori genome. Spatio-temporal expression profiles showed that BmUFL1 expression was high in the midgut, epidermis, and testis and in the pupa-adult stage. BmUFL1 knockdown inhibited B. mori nucleopolyhedrovirus (BmNPV) proliferation, while BmUFL1 overexpression promoted BmNPV proliferation. Mechanically, protein kinase RNA-like endoplasmic reticulum kinase (PERK) signaling and cell apoptosis are involved in BmUFL1-regulated BmNPV proliferation. Overall, these results suggest that BmUFL1 facilitates BmNPV proliferation in silkworm.<br /> (© 2024 The Author(s). Archives of Insect Biochemistry and Physiology published by Wiley Periodicals LLC.)
- Subjects :
- Animals
Virus Replication
Ubiquitin-Protein Ligases metabolism
Ubiquitin-Protein Ligases genetics
Larva virology
Larva growth & development
Larva metabolism
Larva genetics
Bombyx virology
Bombyx genetics
Bombyx growth & development
Apoptosis
Nucleopolyhedroviruses physiology
Insect Proteins metabolism
Insect Proteins genetics
eIF-2 Kinase metabolism
eIF-2 Kinase genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6327
- Volume :
- 116
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Archives of insect biochemistry and physiology
- Publication Type :
- Academic Journal
- Accession number :
- 38976652
- Full Text :
- https://doi.org/10.1002/arch.22127