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Stereospecific reduction of 2'S-configured strigolactones by cowpea OPR3 enzymes.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2024 Sep 20; Vol. 88 (10), pp. 1172-1179. - Publication Year :
- 2024
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Abstract
- Strigolactones (SLs), plant-derived apocarotenoids, serve dual roles as phytohormones and rhizosphere signaling molecules. While exogenous administration of SLs to plants aids in studying their functions, the metabolic destiny of these administered SLs remains poorly elucidated. Our previous research demonstrated that among synthetic SL GR24 stereoisomers administered to cowpea (Vigna unguiculata), 2'-epi-GR24 undergoes selective reduction at the C-3',4' double bond in its D-ring. In this investigation, we isolated proteins from cowpea roots based on SL reducing activity and identified 12-oxophytodienoate reductase 3 homologs (VuOPR3s) as contributors to this reduction. Enzymatic assays conducted with recombinant proteins revealed that VuOPR3s exhibited a preference for reducing activity toward 2'S-configured SLs, including 2'-epi-GR24. This specificity for 2'S-configured SLs was congruent with that observed for orobanchol produced by cowpea and its stereoisomers. These findings suggest that exogenously administered SLs undergo enzymatic stereoselective reduction, underscoring the importance of considering stereospecificity when interpreting data obtained from SL usage.<br /> (© The Author(s) 2024. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)
- Subjects :
- Stereoisomerism
Plant Proteins chemistry
Plant Proteins metabolism
Plant Roots metabolism
Plant Roots chemistry
Substrate Specificity
Recombinant Proteins metabolism
Recombinant Proteins chemistry
Heterocyclic Compounds, 3-Ring
Lactones chemistry
Lactones metabolism
Vigna enzymology
Oxidation-Reduction
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 88
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38970383
- Full Text :
- https://doi.org/10.1093/bbb/zbae097