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A functionalized cell membrane biomimetic nanoformulation based on layered double hydroxide for combined tumor chemotherapy and sonodynamic therapy.
- Source :
-
Journal of materials chemistry. B [J Mater Chem B] 2024 Jul 31; Vol. 12 (30), pp. 7429-7439. Date of Electronic Publication: 2024 Jul 31. - Publication Year :
- 2024
-
Abstract
- The development of nanoformulations with simple compositions that can exert targeted combination therapy still remains a great challenge in the area of precision cancer nanomedicine. Herein, we report the design of a multifunctional nanoplatform based on methotrexate (MTX)-loaded layered double hydroxide (LDH) coated with chlorin e6 (Ce6)-modified MCF-7 cell membranes (CM <subscript>M</subscript> ) for combined chemo/sonodynamic therapy of breast cancer. LDH nanoparticles were in situ loaded with MTX via coprecipitation, and coated with CM <subscript>M</subscript> that were finally functionalized with phospholipid-modified Ce6. The created nanoformulation of LDH-MTX@CM <subscript>M</subscript> -Ce6 displays good colloidal stability under physiological conditions and can release MTX in a pH-dependent manner. We show that the formulation can homologously target breast cancer cells, and induce their significant apoptosis through arresting the cell cycle via cooperative MTX-based chemotherapy and ultrasound (US)-activated sonodynamic therapy. The assistance of US can not only trigger sonosensitizer Ce6 to produce reactive oxygen species, but also enhance the cellular uptake of LDH-MTX@CM <subscript>M</subscript> -Ce6 via an acoustic cavitation effect. Upon intravenous injection and US irradiation, LDH-MTX@CM <subscript>M</subscript> -Ce6 displays an admirable antitumor performance towards a xenografted breast tumor mouse model. Furthermore, the modification of Ce6 on the CM <subscript>M</subscript> endows the LDH-based nanoplatform with fluorescence imaging capability. The developed LDH-based nanoformulation here provides a general intelligent cancer nanomedicine platform with simple composition and homologous targeting specificity for combined chemo/sonodynamic therapy and fluorescence imaging of tumors.
- Subjects :
- Humans
Animals
Mice
Female
MCF-7 Cells
Antineoplastic Agents chemistry
Antineoplastic Agents pharmacology
Apoptosis drug effects
Cell Membrane chemistry
Cell Membrane metabolism
Mice, Inbred BALB C
Biomimetic Materials chemistry
Biomimetic Materials pharmacology
Mice, Nude
Cell Proliferation drug effects
Breast Neoplasms drug therapy
Breast Neoplasms pathology
Particle Size
Drug Screening Assays, Antitumor
Surface Properties
Methotrexate chemistry
Methotrexate pharmacology
Hydroxides chemistry
Hydroxides pharmacology
Chlorophyllides
Porphyrins chemistry
Porphyrins pharmacology
Nanoparticles chemistry
Ultrasonic Therapy
Subjects
Details
- Language :
- English
- ISSN :
- 2050-7518
- Volume :
- 12
- Issue :
- 30
- Database :
- MEDLINE
- Journal :
- Journal of materials chemistry. B
- Publication Type :
- Academic Journal
- Accession number :
- 38967310
- Full Text :
- https://doi.org/10.1039/d4tb00813h