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Autologous bone marrow transplantation with marrow decontaminated by immunotoxin T 101 in the treatment of leukemia and lymphoma: first clinical observations.
- Source :
-
Cancer treatment reports [Cancer Treat Rep] 1985 Sep; Vol. 69 (9), pp. 953-9. - Publication Year :
- 1985
-
Abstract
- Four patients with T-cell malignancies of poor prognosis (three with non-Hodgkin's lymphoma and one with acute lymphoblastic leukemia) received the following consolidation therapy for complete or partial remission: cyclophosphamide (120 mg/kg) plus total-body irradiation, followed by reinfusion of cryopreserved autologous marrow previously purged in vitro by immunotoxin T 101 (SR 41322). This immunotoxin is made of the murine monoclonal T 101 antibody coupled to chain A of ricin. The doses of immunotoxin used were 10(-9) and 10(-8) M, and the durations of incubation were 4 and 20 hours at 37 degrees C. Recovery of progenitors CFUc and BFUe was total following incubation with immunotoxin T 101, but diminished after cryopreservation (15%-80% for CFUc, 33%-47% for BFUe), suggesting an increased fragility of the incubated progenitors to freezing. In every case, hematopoietic recovery occurred within normal time periods, with a wbc count greater than 10(9)/L and a platelet count greater than 50 X 10(9)/L on Day 22 (range, 15-31) and Day 21 (range, 22-47), respectively, demonstrating the feasibility of autologous bone marrow transplantation with marrow pretreated by immunotoxin. However, the slow recovery of lymphocytes and the development of severe infections in two patients may indicate that an in-depth study of immunological reconstitution after in vitro treatment of the marrow with immunotoxin T 101 is necessary.
- Subjects :
- Adolescent
Adult
Antibodies, Monoclonal adverse effects
Antibodies, Monoclonal therapeutic use
Antineoplastic Combined Chemotherapy Protocols therapeutic use
Bone Marrow Transplantation
Combined Modality Therapy
Cyclophosphamide administration & dosage
Cytarabine administration & dosage
Cytotoxicity, Immunologic
Drug Evaluation
Female
Hematopoietic Stem Cells drug effects
Hematopoietic Stem Cells immunology
Humans
Leukemia, Lymphoid blood
Leukemia, Lymphoid mortality
Lymphoma blood
Lymphoma mortality
Male
Mediastinal Neoplasms therapy
Prognosis
Ricin adverse effects
Ricin therapeutic use
Specimen Handling
Whole-Body Irradiation
Antibodies, Monoclonal immunology
Bone Marrow immunology
Leukemia, Lymphoid therapy
Lymphoma therapy
Ricin immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0361-5960
- Volume :
- 69
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Cancer treatment reports
- Publication Type :
- Academic Journal
- Accession number :
- 3896482