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A universal fluorescence biosensor based on rolling circle amplification and locking probe for DNA detection.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2024 Jul 01; Vol. 191 (7), pp. 437. Date of Electronic Publication: 2024 Jul 01. - Publication Year :
- 2024
-
Abstract
- A stable DNA signal amplification sensor was developed on account of rolling circle amplification (RCA). This sensor includes target DNA-controlled rolling circle amplification technology and locking probe DNA replacement technology, which can be used to detect DNA fragments with genetic information, thus constructing a biosensor for universal detection of DNA. This study takes the homologous DNA of human immunodeficiency virus (HIV) and let-7a as examples to describe this biosensor. The padlock probe is first cyclized by T4 DNA ligase in response to the target's reaction with it. Then, rolling cycle amplification is initiated by Phi29 DNA polymerase, resulting in the formation of a lengthy chain with several triggers. These triggers can open the locked probe LP1 with the fluorescence signal turned off, so that it can continue to react with H2 to form a stable H1-H2 double strand. This regulates the distance between B-DNA modified by the quenching group and H1 modified by fluorescent group, and the fluorescence signal is recovered.<br /> (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
- Subjects :
- Humans
Fluorescent Dyes chemistry
DNA, Viral analysis
DNA, Viral genetics
DNA chemistry
DNA genetics
Spectrometry, Fluorescence methods
Fluorescence
DNA-Directed DNA Polymerase metabolism
DNA-Directed DNA Polymerase chemistry
Limit of Detection
HIV genetics
Biosensing Techniques methods
Nucleic Acid Amplification Techniques methods
DNA Probes chemistry
DNA Probes genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 191
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 38951284
- Full Text :
- https://doi.org/10.1007/s00604-024-06501-2