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Visualization of cysteine in AD mouse with a high-quantum yield NIR fluorescent probe.
- Source :
-
Talanta [Talanta] 2024 Oct 01; Vol. 278, pp. 126482. Date of Electronic Publication: 2024 Jun 26. - Publication Year :
- 2024
-
Abstract
- Alzheimer's disease (AD) has gradually received enthusiastic attention with the aging process, and studying its biological relevance is expected. Excitingly, fluorescence probes were considered to be powerful tools for exploring biological correlations. Therefore, a highly selective near-infrared (NIR) fluorescent probe (DCM-Cl-Acr) for imaging cysteine (Cys) in AD was designed and synthesized. Through structural optimization, the probe exhibited high fluorescence quantum yield and low detection limit (20 nM) towards Cys. Meanwhile, based on the high selectivity and high sensitivity response exhibited by the probe to Cys, it was successfully applied to visualize endogenous and exogenous Cys in living cells and zebrafish, and showed good discrimination from homocysteine (Hcy) and glutathione (GSH). Further, the correlation between AD and Cys concentration was clarified by imaging studies in hippocampus tissue of AD mouse, and the abnormal accumulation of Cys in the hippocampus of AD brain was demonstrated.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Mice
Humans
Hippocampus diagnostic imaging
Hippocampus metabolism
Hippocampus chemistry
Optical Imaging methods
Infrared Rays
Cysteine analysis
Cysteine chemistry
Fluorescent Dyes chemistry
Fluorescent Dyes chemical synthesis
Alzheimer Disease diagnostic imaging
Alzheimer Disease metabolism
Alzheimer Disease diagnosis
Zebrafish
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3573
- Volume :
- 278
- Database :
- MEDLINE
- Journal :
- Talanta
- Publication Type :
- Academic Journal
- Accession number :
- 38950502
- Full Text :
- https://doi.org/10.1016/j.talanta.2024.126482