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Transcriptome profiling of fast/glycolytic and slow/oxidative muscle fibers in aging and obesity.

Authors :
Zhang FM
Wu HF
Wang KF
Yu DY
Zhang XZ
Ren Q
Chen WZ
Lin F
Yu Z
Zhuang CL
Source :
Cell death & disease [Cell Death Dis] 2024 Jun 28; Vol. 15 (6), pp. 459. Date of Electronic Publication: 2024 Jun 28.
Publication Year :
2024

Abstract

Aging and obesity pose significant threats to public health and are major contributors to muscle atrophy. The trends in muscle fiber types under these conditions and the transcriptional differences between different muscle fiber types remain unclear. Here, we demonstrate distinct responses of fast/glycolytic fibers and slow/oxidative fibers to aging and obesity. We found that in muscles dominated by oxidative fibers, the proportion of oxidative fibers remains unchanged during aging and obesity. However, in muscles dominated by glycolytic fibers, despite the low content of oxidative fibers, a significant decrease in proportion of oxidative fibers was observed. Consistently, our study uncovered that during aging and obesity, fast/glycolytic fibers specifically increased the expression of genes associated with muscle atrophy and inflammation, including Dkk3, Ccl8, Cxcl10, Cxcl13, Fbxo32, Depp1, and Chac1, while slow/oxidative fibers exhibit elevated expression of antioxidant protein Nqo-1 and downregulation of Tfrc. Additionally, we noted substantial differences in the expression of calcium-related signaling pathways between fast/glycolytic fibers and slow/oxidative fibers in response to aging and obesity. Treatment with a calcium channel inhibitor thapsigargin significantly increased the abundance of oxidative fibers. Our study provides additional evidence to support the transcriptomic differences in muscle fiber types under pathophysiological conditions, thereby establishing a theoretical basis for modulating muscle fiber types in disease treatment.<br /> (© 2024. The Author(s).)

Details

Language :
English
ISSN :
2041-4889
Volume :
15
Issue :
6
Database :
MEDLINE
Journal :
Cell death & disease
Publication Type :
Academic Journal
Accession number :
38942747
Full Text :
https://doi.org/10.1038/s41419-024-06851-y