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Teleost-specific TLR23 in Takifugu rubripes recruits MyD88 to trigger ERK pathway and promotes antibacterial defense.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Aug; Vol. 151, pp. 109724. Date of Electronic Publication: 2024 Jun 26. - Publication Year :
- 2024
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Abstract
- Takifugu rubripes is a highly valued cultured fish in Asia, while pathogen infections can result in severe diseases and lead to substantial economic losses. Toll-like receptors (TLRs), as pattern recognition receptors, play a crucial role on recognition pathogens and initiation innate immune response. However, the immunological properties of teleost-specific TLR23 remain largely unknown. In this study, we investigated the biological functions of TLR23 (TrTLR23) from T. rubripes, found that TrTLR23 existed in various organs. Following bacterial pathogen challenge, the expression levels of TrTLR23 were significantly increased in immune related organs. TrTLR23 located on the cellular membrane and specifically recognized pathogenic microorganism. Co-immunoprecipitation and antibody blocking analysis revealed that TrTLR23 recruited myeloid differentiation primary response protein (MyD88), thereby mediating the activation of the ERK signaling pathway. Furthermore, in vivo showed that, when TrTLR23 is overexpressed in T. rubripes, bacterial replication in fish tissues is significantly inhibited. Consistently, when TrTLR23 expression in T. rubripes is knocked down, bacterial replication is significantly enhanced. In conclusion, these findings suggested that TrTLR23 played a critical role on mediation TLR23-MyD88-ERK axis against bacterial infection. This study revealed that TLR23 involved in the innate immune mechanism, and provided the foundation for development disease control strategies in teleost.<br />Competing Interests: Declaration of competing interest The authors declare no competing interests.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
MAP Kinase Signaling System immunology
Gene Expression Regulation immunology
Edwardsiella physiology
Edwardsiella immunology
Vibrio physiology
Fish Proteins genetics
Fish Proteins immunology
Takifugu immunology
Takifugu genetics
Fish Diseases immunology
Myeloid Differentiation Factor 88 genetics
Myeloid Differentiation Factor 88 metabolism
Myeloid Differentiation Factor 88 immunology
Immunity, Innate genetics
Toll-Like Receptors genetics
Toll-Like Receptors immunology
Toll-Like Receptors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 151
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38942251
- Full Text :
- https://doi.org/10.1016/j.fsi.2024.109724