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Lens placode modulates extracellular matrix formation during early eye development.
- Source :
-
Differentiation; research in biological diversity [Differentiation] 2024 Jul-Aug; Vol. 138, pp. 100792. Date of Electronic Publication: 2024 Jun 22. - Publication Year :
- 2024
-
Abstract
- The role extracellular matrix (ECM) in multiple events of morphogenesis has been well described, little is known about its specific role in early eye development. One of the first morphogenic events in lens development is placodal thickening, which converts the presumptive lens ectoderm from cuboidal to pseudostratified epithelium. This process occurs in the anterior pre-placodal ectoderm when the optic vesicle approaches the cephalic ectoderm and is regulated by transcription factor Pax6 and secreted BMP4. Since cells and ECM have a dynamic relationship of interdependence and modulation, we hypothesized that the ECM evolves with cell shape changes during lens placode formation. This study investigates changes in optic ECM including both protein distribution deposition, extracellular gelatinase activity and gene expression patterns during early optic development using chicken and mouse models. In particular, the expression of Timp2, a metalloprotease inhibitor, corresponds with a decrease in gelatinase activity within the optic ECM. Furthermore, we demonstrate that optic ECM remodeling depends on BMP signaling in the placode. Together, our findings suggest that the lens placode plays an active role in remodeling the optic ECM during early eye development.<br /> (Copyright © 2024 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Mice
Eye Proteins metabolism
Eye Proteins genetics
Bone Morphogenetic Protein 4 metabolism
Bone Morphogenetic Protein 4 genetics
Chick Embryo
Homeodomain Proteins metabolism
Homeodomain Proteins genetics
Tissue Inhibitor of Metalloproteinase-2 metabolism
Tissue Inhibitor of Metalloproteinase-2 genetics
Paired Box Transcription Factors metabolism
Paired Box Transcription Factors genetics
Repressor Proteins metabolism
Repressor Proteins genetics
Signal Transduction
Chickens genetics
Eye metabolism
Eye growth & development
Eye embryology
Extracellular Matrix metabolism
Lens, Crystalline metabolism
Lens, Crystalline growth & development
Lens, Crystalline cytology
PAX6 Transcription Factor metabolism
PAX6 Transcription Factor genetics
Gene Expression Regulation, Developmental
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0436
- Volume :
- 138
- Database :
- MEDLINE
- Journal :
- Differentiation; research in biological diversity
- Publication Type :
- Academic Journal
- Accession number :
- 38935992
- Full Text :
- https://doi.org/10.1016/j.diff.2024.100792