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The Effect of Targeted Hyperoxemia on Brain Immunohistochemistry after Long-Term, Resuscitated Porcine Acute Subdural Hematoma and Hemorrhagic Shock.
- Source :
-
International journal of molecular sciences [Int J Mol Sci] 2024 Jun 14; Vol. 25 (12). Date of Electronic Publication: 2024 Jun 14. - Publication Year :
- 2024
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Abstract
- Epidemiological data suggest that moderate hyperoxemia may be associated with an improved outcome after traumatic brain injury. In a prospective, randomized investigation of long-term, resuscitated acute subdural hematoma plus hemorrhagic shock (ASDH + HS) in 14 adult, human-sized pigs, targeted hyperoxemia (200 < P <subscript>a</subscript> O <subscript>2</subscript> < 250 mmHg vs. normoxemia 80 < P <subscript>a</subscript> O <subscript>2</subscript> < 120 mmHg) coincided with improved neurological function. Since brain perfusion, oxygenation and metabolism did not differ, this post hoc study analyzed the available material for the effects of targeted hyperoxemia on cerebral tissue markers of oxidative/nitrosative stress (nitrotyrosine expression), blood-brain barrier integrity (extravascular albumin accumulation) and fluid homeostasis (oxytocin, its receptor and the H <subscript>2</subscript> S-producing enzymes cystathionine-β-synthase and cystathionine-γ-lyase). After 2 h of ASDH + HS (0.1 mL/kgBW autologous blood injected into the subdural space and passive removal of 30% of the blood volume), animals were resuscitated for up to 53 h by re-transfusion of shed blood, noradrenaline infusion to maintain cerebral perfusion pressure at baseline levels and hyper-/normoxemia during the first 24 h. Immediate postmortem, bi-hemispheric (i.e., blood-injected and contra-lateral) prefrontal cortex specimens from the base of the sulci underwent immunohistochemistry (% positive tissue staining) analysis of oxidative/nitrosative stress, blood-brain barrier integrity and fluid homeostasis. None of these tissue markers explained any differences in hyperoxemia-related neurological function. Likewise, hyperoxemia exerted no deleterious effects.
- Subjects :
- Animals
Swine
Blood-Brain Barrier metabolism
Immunohistochemistry
Oxidative Stress
Resuscitation methods
Disease Models, Animal
Oxygen metabolism
Tyrosine analogs & derivatives
Tyrosine metabolism
Hematoma, Subdural, Acute metabolism
Hematoma, Subdural, Acute etiology
Hematoma, Subdural, Acute pathology
Shock, Hemorrhagic metabolism
Brain metabolism
Brain pathology
Subjects
Details
- Language :
- English
- ISSN :
- 1422-0067
- Volume :
- 25
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- International journal of molecular sciences
- Publication Type :
- Academic Journal
- Accession number :
- 38928283
- Full Text :
- https://doi.org/10.3390/ijms25126574