Back to Search Start Over

Tailoring silk fibroin fibrous architecture by a high-yield electrospinning method for fast wound healing possibilities.

Authors :
Zhu JC
Wang H
Wu CX
Zhang KQ
Ye H
Source :
Biotechnology and bioengineering [Biotechnol Bioeng] 2024 Oct; Vol. 121 (10), pp. 3224-3238. Date of Electronic Publication: 2024 Jun 25.
Publication Year :
2024

Abstract

In this study, a novel array electrospinning collector was devised to generate two distinct regenerated silk fibroin (SF) fibrous membranes: ordered and disordered. Leveraging electrostatic forces during the electrospinning process allowed precise control over the orientation of SF fiber, resulting in the creation of membranes comprising both aligned and randomly arranged fiber layers. This innovative approach resulted in the development of large-area membranes featuring exceptional stability due to their alternating patterned structure, achievable through expansion using the collector, and improving the aligned fiber membrane mechanical properties. The study delved into exploring the potential of these membranes in augmenting wound healing efficiency. Conducting in vitro toxicity assays with adipose tissue-derived mesenchymal stem cells (AD-MSCs) and normal human dermal fibroblasts (NHDFs) confirmed the biocompatibility of the SF membranes. We use dual perspectives on exploring the effects of different conditioned mediums produced by cells and structural cues of materials on NHDFs migration. The nanofibers providing the microenvironment can directly guide NHDFs migration and also affect the AD-MSCs and NHDFs paracrine effects, which can improve the chemotaxis of NHDFs migration. The ordered membrane, in particular, exhibited pronounced effectiveness in guiding directional cell migration. This research underscores the revelation that customizable microenvironments facilitated by SF membranes optimize the paracrine products of mesenchymal stem cells and offer valuable physical cues, presenting novel prospects for enhancing wound healing efficiency.<br /> (© 2024 The Author(s). Biotechnology and Bioengineering published by Wiley Periodicals LLC.)

Details

Language :
English
ISSN :
1097-0290
Volume :
121
Issue :
10
Database :
MEDLINE
Journal :
Biotechnology and bioengineering
Publication Type :
Academic Journal
Accession number :
38924076
Full Text :
https://doi.org/10.1002/bit.28783