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Comparative studies on the intestinal health of wild and cultured ricefield eel ( Monopterus albus ).

Authors :
Yang H
Yuan Q
Rahman MM
Lv W
Huang W
Hu W
Zhou W
Source :
Frontiers in immunology [Front Immunol] 2024 Jun 10; Vol. 15, pp. 1411544. Date of Electronic Publication: 2024 Jun 10 (Print Publication: 2024).
Publication Year :
2024

Abstract

Fish intestinal health under intensive aquaculture mode plays an important role in growth, development, and immune function. The present study was aimed to systematically investigate the differences of intestinal health between wild and cultured Monopterus albus by biochemical parameters, histomorphology, and molecular biology. A total of 15 healthy M. albus per group, with an average body weight of 45 g, were sampled to analyze intestinal health parameters. Compared with wild fish, the cultured M. albus in the foregut had lower trypsin, lipase, SOD, CAT, T-AOC, and GSH-Px activities ( P < 0.05) and higher amylase activity and MDA content ( P < 0.05). The villus circumference and goblet cells in the cultured group were significantly lower than those in the wild group ( P < 0.05). In addition, the cultured fish showed lower relative expression levels of occludin , zo-1 , zo-2 , claudin-12 , claudin-15 , mucin5 , mucin15 , lysozyme , complement 3 , il-10 , tgf-β1 , tgf-β2 , and tgf-β3 ( P < 0.05) and higher il-1β , il-6 , il-8 , tnf-a , and ifnγ mRNA expressions than those of wild fish ( P < 0.05). In terms of gut microbiota, the cultured group at the phylum level displayed higher percentages of Chlamydiae and Spirochaetes and lower percentages of Firmicutes , Bacteroidetes , Actinobacteria , Cyanobacteria , and Verrucomicrobia compared to the wild group ( P < 0.05). At the genus level, higher abundances of Pseudomonadaceae_Pseudomonas and Spironema and lower abundances of Lactococcus and Cetobacterium were observed in the cultured group than in the wild group ( P < 0.05). To our knowledge, this is the first investigation of the intestinal health status between wild and cultured M. albus in terms of biochemistry, histology, and molecular biology levels. Overall, the present study showed significant differences in intestinal health between wild and cultured M. albus and the main manifestations that wild M. albus had higher intestinal digestion, antioxidant capacity, and intestinal barrier functions than cultured M. albus. These results would provide theoretical basis for the subsequent upgrading of healthy aquaculture technology and nutrient regulation of intestinal health of cultured M. albus.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2024 Yang, Yuan, Rahman, Lv, Huang, Hu and Zhou.)

Details

Language :
English
ISSN :
1664-3224
Volume :
15
Database :
MEDLINE
Journal :
Frontiers in immunology
Publication Type :
Academic Journal
Accession number :
38915412
Full Text :
https://doi.org/10.3389/fimmu.2024.1411544