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Essential oil from Chimonanthus nitens Oliv. Leaves ameliorate inflammation and oxidative stress in LPS-induced ALI through NF-κB and Nrf2 signaling pathways.
- Source :
-
Journal of ethnopharmacology [J Ethnopharmacol] 2024 Oct 28; Vol. 333, pp. 118470. Date of Electronic Publication: 2024 Jun 21. - Publication Year :
- 2024
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Abstract
- Ethnopharmacological Relevance: Initial investigative research indicated that the essential oil from Chimonanthus nitens Oliv. Leaves (CLO) significantly reduces lung tissues inflammation and effectively repairs Acute lung injury (ALI) mice model. However, the mechanism underlying is not clear, and the impacts of CLO on oxidative stress require further investigation.<br />Aim of the Study: The purpose of the experiment was to validate the influence of CLO in ALI model mice, as well as its potential mechanisms.<br />Materials and Methods: Lipopolysaccharide-induced establishment of the A549 cell inflammation model, and ALI mice model was established by intrathecal administration of LPS.<br />Results: CLO significantly reduced the release of inflammatory cytokines in A549 cells, lowered MDA and ROS levels, and enhanced SOD activity. Animal experiment results showed that CLO dramatically decreased white blood cell count, the expression of inflammatory cytokines, and the destruction of alveolar structures. CLO enhances the activity of antioxidant enzymes. Western Blot and q-PCR analyses have revealed that the mechanism of CLO is correlation with the NF-κB and Nrf2 signaling pathways in cellular and animal models. Pathway inhibitor experiments indicated that there might be functional crosstalk between these two pathways.<br />Conclusions: CLO may regulate inflammation and oxidative stress in LPS-induced ALI through NF-κB and Nrf2 signaling pathways. This finding could be novel in the pharmacological treatment of ALI.<br />Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Humans
Male
Mice
A549 Cells
Anti-Inflammatory Agents pharmacology
Anti-Inflammatory Agents isolation & purification
Calycanthaceae chemistry
Cytokines metabolism
Disease Models, Animal
Inflammation drug therapy
Inflammation chemically induced
NF-E2-Related Factor 2 metabolism
NF-kappa B metabolism
Acute Lung Injury drug therapy
Acute Lung Injury chemically induced
Acute Lung Injury metabolism
Lipopolysaccharides
Oils, Volatile pharmacology
Oxidative Stress drug effects
Plant Leaves chemistry
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1872-7573
- Volume :
- 333
- Database :
- MEDLINE
- Journal :
- Journal of ethnopharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 38909829
- Full Text :
- https://doi.org/10.1016/j.jep.2024.118470