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Goose IFIT5 positively regulates goose astrovirus replication in GEF cells.
- Source :
-
Poultry science [Poult Sci] 2024 Aug; Vol. 103 (8), pp. 103930. Date of Electronic Publication: 2024 Jun 01. - Publication Year :
- 2024
-
Abstract
- Interferon-induced protein with tetratricopeptide repeats (IFITs), a family of proteins strongly induced by type I interferon (IFN-I), are deeply involved in many cellular and viral processes. IFIT5, the sole protein in this family found in birds, also plays a crucial role in regulating virus infection. In this study, goose IFIT5 (gIFIT5) was first cloned from peripheral blood lymphocyte (PBL) and phylogenetic analysis showed that it was highly homologous with duck IFIT5 (dIFIT5), sharing 94.6% identity in amino acid sequence. Subsequently, the expression kinetics of gIFIT5 during goose astrovirus (GAstV) infection and the regulatory effect of gIFIT5 on GAstV proliferation were evaluated. Results showed that the mRNA and protein expression level of gIFIT5 was greatly induced by GAstV infection, especially at 12 hpi. Importantly, gIFIT5 could conversely promote GAstV replication in GEF cells. Virus titers in gIFIT5 overexpression group were significantly higher than those in control group at 12 and 24 hpi. Western blot and quantitative real-time PCR (qRT-PCR) further demonstrated that the production of viral cap protein was significantly facilitated in gIFIT5-transfected group. Collectively, GAstV facilitates self-replication via promoting gIFIT5 expression.<br />Competing Interests: DISCLOSURES The authors declare no conflict of interest.<br /> (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Animals
Phylogeny
Avastrovirus physiology
Avastrovirus genetics
Amino Acid Sequence
Gene Expression Regulation
Geese physiology
Geese virology
Virus Replication
Astroviridae Infections veterinary
Astroviridae Infections virology
Poultry Diseases virology
Avian Proteins genetics
Avian Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1525-3171
- Volume :
- 103
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Poultry science
- Publication Type :
- Academic Journal
- Accession number :
- 38908126
- Full Text :
- https://doi.org/10.1016/j.psj.2024.103930