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Prevalence and characterization of foodborne pathogens isolated from fresh-cut fruits and vegetables in Beijing, China.
- Source :
-
International journal of food microbiology [Int J Food Microbiol] 2024 Aug 16; Vol. 421, pp. 110804. Date of Electronic Publication: 2024 Jun 17. - Publication Year :
- 2024
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Abstract
- Pre-cut fresh fruits and vegetables are highly appealing to consumers for their convenience, however, as they are highly susceptible to microbial contamination in processing, the potential risks of foodborne illnesses to public health are not negligible. This study aimed to assess the prevalence, antibiotic susceptibility and molecular characteristics of major foodborne pathogens (Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella) isolated from fresh-cut fruits and vegetables in Beijing, China. 86 stains were isolated from 326 samples, with S. aureus being the highest prevalence (15.38 %), followed by E. coli (9.23 %) and L. monocytogenes (1.85 %), while no Salmonella was detected. The prevalence by type of food indicated that fruit trays and mixed vegetables were more susceptible to contamination by pathogens. 98 % of S. aureus were resistant to at least of one antibiotic, and showed a high resistance rate to benzylpenicillin (90 %) and oxacillin (48 %). Among 25 E. coli isolates, 57.67 % of which exhibited multi-drug resistance, with common resist to trimethoprim/sulfamethoxazole (66.67 %) and ampicillin (63.33 %). A total of 9 sequence types (STs) and 8 spa types were identified in 35 S. aureus isolates, with ST398-t34 being the predominant type (42.86 %). Additionally, analysis of 25 E. coli isolates demonstrated significant heterogeneity, characterized by 22 serotypes and 18 STs. Genomic analysis revealed that 5 and 44 distinct antibiotic resistance genes (ARGs) in S. aureus and E. coli, respectively. Seven quinolone resistance-determining regions (QRDRs) mutations were identified in E. coli isolates, of which GyrA (S83L) was the most frequently detected. All the S. aureus and E. coli isolates harbored virulence genes. ARGs in S. aureus and E. coli showed a significant positive correlation with plasmids. Furthermore, one L. monocytogenes isolate, which was ST101 and serogroupIIc from watermelon sample, harbored virulence genes (inlA and inlB) and LIPI-1 pathogenic islands (prfA, plcA, hly and actA), which posed potential risks for consumer's health. This study focused on the potential microbial risk of fresh-cut fruits and vegetables associated with foodborne diseases, improving the scientific understanding towards risk assessment related to ready-to-eat foods.<br />Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Subjects :
- Beijing epidemiology
Salmonella genetics
Salmonella isolation & purification
Salmonella classification
Salmonella drug effects
Prevalence
Food Contamination analysis
China epidemiology
Listeria monocytogenes genetics
Listeria monocytogenes isolation & purification
Listeria monocytogenes classification
Listeria monocytogenes drug effects
Drug Resistance, Bacterial genetics
Drug Resistance, Multiple, Bacterial genetics
Foodborne Diseases microbiology
Foodborne Diseases epidemiology
Vegetables microbiology
Fruit microbiology
Staphylococcus aureus genetics
Staphylococcus aureus isolation & purification
Staphylococcus aureus drug effects
Anti-Bacterial Agents pharmacology
Food Microbiology
Escherichia coli isolation & purification
Escherichia coli genetics
Escherichia coli drug effects
Microbial Sensitivity Tests
Subjects
Details
- Language :
- English
- ISSN :
- 1879-3460
- Volume :
- 421
- Database :
- MEDLINE
- Journal :
- International journal of food microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 38905809
- Full Text :
- https://doi.org/10.1016/j.ijfoodmicro.2024.110804