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Shenqi Pill Mitigates Renal Interstitial Fibrosis Through Weakening Notch1/Jag1 Pathway.
- Source :
-
Iranian journal of kidney diseases [Iran J Kidney Dis] 2024 May; Vol. 18 (3), pp. 159-167. - Publication Year :
- 2024
-
Abstract
- Introduction: Shenqi pill (SQP) can be used to treat various kidney related diseases, but its exact mechanism of action remains unclear. We intended to analyze the role and mechanism of SQP on renal interstitial fibrosis (RIF).<br />Methods: After performing unilateral ureteral obstruction (UUO) surgery following the Institutional Animal Care and Use Committee guidelines, all rats were assigned into the sham group, UUO group, UUO + SQP 1.5 g/kg, UUO + SQP 3 g/kg, and UUO + SQP 6 g/kg groups. After treatment with SQP for 4 weeks, the appearance of kidney, serum creatinine (SCr), and blood urea nitrogen (BUN) levels were monitored in each group. The pathological injury, extracellular matrix (ECM), and Notch1 pathway-related protein levels were measured using H&E staining, Masson staining, immunohistochemistry, and Western blot, respectively.<br />Results: SQP could obviously ameliorate the appearance of the kidney as well as the levels of SCr and BUN in UUO rats (SCr: 67.6 ± 4.64 μM, 59.66 ± 4.96 μM, 48.76 ± 4.44 μM, 40.43 ± 3.02 μM for UUO, low, medium, and high SQP treatment groups; BUN: 9.09 ± 0.97 mM, 7.72 ± 0.61 mM, 5.42 ± 0.42 mM, 4.24 ± 0.34 mM for UUO, low, medium, and high SQP treatment groups; P < .05). SQP also effectively mitigated renal tissue injury in UUO rats (P < .05). Moreover, we uncovered that SQP significantly inhibited Collagen I, α-SMA, Collagen IV, TGF-B1, Notch1, and Jag1 protein expressions in UUO rats kidney (P < .05).<br />Conclusion: Our data elucidated that SQP can alleviate RIF, and the mechanism may be related to the Notch1/Jag1 pathway. DOI: 10.52547/ijkd.7703.
- Subjects :
- Animals
Male
Rats
Disease Models, Animal
Kidney Diseases pathology
Kidney Diseases drug therapy
Kidney Diseases prevention & control
Kidney Diseases metabolism
Creatinine blood
Transforming Growth Factor beta1 metabolism
Actins metabolism
Drugs, Chinese Herbal pharmacology
Fibrosis
Receptor, Notch1 metabolism
Kidney pathology
Kidney drug effects
Kidney metabolism
Ureteral Obstruction drug therapy
Ureteral Obstruction complications
Ureteral Obstruction pathology
Rats, Sprague-Dawley
Signal Transduction drug effects
Jagged-1 Protein metabolism
Blood Urea Nitrogen
Subjects
Details
- Language :
- English
- ISSN :
- 1735-8604
- Volume :
- 18
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Iranian journal of kidney diseases
- Publication Type :
- Academic Journal
- Accession number :
- 38904340
- Full Text :
- https://doi.org/10.52547/b56av842