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Norepinephrine regulates TNF expression via the A1AR-p38 MAPK-Relish pathway in granulocytes of oyster Crassostrea gigas.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2024 Oct; Vol. 159, pp. 105217. Date of Electronic Publication: 2024 Jun 18. - Publication Year :
- 2024
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Abstract
- Norepinephrine (NE) is involved in regulating cytokine expression and phagocytosis of immune cells in the innate immunity of vertebrates. In the present study, the modulation mechanism of NE on the biosynthesis of TNFs in oyster granulocytes was explored. The transcripts of CgTNF-1, CgTNF-2 and CgTNF-3 were highly expressed in granulocytes, and they were significantly up-regulated after LPS stimulation, while down-regulated after NE treatment. The phagocytic rate and apoptosis index of oyster granulocytes were also triggered by LPS stimulation and suppressed by NE treatment. The mRNA expressions of CgMAPK14 and CgRelish were significantly induced after NE treatment, and the translocation of CgRelish from cytoplasm to nucleus was observed. The concentration of intracellular Ca <superscript>2+</superscript> in granulocytes was significantly up-regulated upon NE incubation, and this trend reverted after the treatment with DOX (specific antagonist for NE receptor, CgA1AR-1). No obvious significance was observed in intracellular cAMP concentrations in the PBS, NE and NE + DOX groups. Once CgA1AR-1 was blocked by DOX, the mRNA expressions of CgMAPK14 and CgRelish were significantly inhibited, and the translocation of CgRelish from cytoplasm to nucleus was also dramatically suppressed, while the mRNA expression of CgTNF-1 and the apoptosis index increased significantly to the same level with those in LPS group, respectively. These results collectively suggested that NE modulated TNF expression in oyster granulocyte through A1AR-p38 MAPK-Relish signaling pathway.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Apoptosis
Signal Transduction
Phagocytosis
Cells, Cultured
Tumor Necrosis Factor-alpha metabolism
Gene Expression Regulation
MAP Kinase Signaling System immunology
Tumor Necrosis Factors metabolism
Tumor Necrosis Factors genetics
Crassostrea immunology
Norepinephrine metabolism
Norepinephrine pharmacology
Granulocytes immunology
Granulocytes metabolism
p38 Mitogen-Activated Protein Kinases metabolism
Immunity, Innate
Lipopolysaccharides immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 159
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38901503
- Full Text :
- https://doi.org/10.1016/j.dci.2024.105217