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Lactobacillus plantarum AR495 improves colonic transport hyperactivity in irritable bowel syndrome through tryptophan metabolism.
- Source :
-
Food & function [Food Funct] 2024 Jul 15; Vol. 15 (14), pp. 7416-7429. Date of Electronic Publication: 2024 Jul 15. - Publication Year :
- 2024
-
Abstract
- Lactobacillus plantarum AR495 is a widely used probiotic for the treatment of various digestive diseases, including irritable bowel syndrome (IBS). However, the specific mechanisms of L. plantarum AR495 in alleviating IBS remain unclear. Abnormal intestinal tryptophan metabolism can cause disordered immune responses, gastrointestinal peristalsis, digestion and sensation, which is closely related to IBS pathogenesis. The aim of this study is to explore the effects and mechanisms of L. plantarum AR495 in regulating tryptophan metabolism. Primarily, tryptophan and its related metabolites in patients with IBS and healthy people were analyzed, and an IBS rat model of acetic acid enema plus restraint stress was established to explore the alleviation pathway of L. plantarum AR495 in tryptophan metabolism. It was found that the 5-HT pathway was significantly changed, and the 5-HTP and 5-HT metabolites were significantly increased in the feces of patients with IBS, which were consistent with the results obtained for the IBS rat model. Maladjusted 5-HT could increase intestinal peristalsis and lead to an increase in the fecal water content and shapeless stool in rats. On the contrary, these two metabolites could be restored to normal levels via intragastric administration of L. plantarum AR495. Further study of the metabolic pathway showed that L. plantarum AR495 could effectively reduce the abundance of 5-HT by inhibiting the expression of enterochromaffin cells rather than promoting its decomposition. In addition, the results showed that L. plantarum AR495 did not affect the expression of SERT. To sum up, L. plantarum AR495 could restore the normal levels of 5-HT by inhibiting the abnormal proliferation of enterochromaffin cells and the excessive activation of TPH1 to inhibit the intestinal peristalsis in IBS. These findings provide insights for the use of probiotics in the treatment of IBS and other diarrheal diseases.
- Subjects :
- Animals
Rats
Humans
Male
Female
Adult
Middle Aged
Disease Models, Animal
Feces microbiology
Young Adult
Irritable Bowel Syndrome metabolism
Irritable Bowel Syndrome therapy
Irritable Bowel Syndrome microbiology
Lactobacillus plantarum metabolism
Tryptophan metabolism
Probiotics pharmacology
Colon metabolism
Colon microbiology
Serotonin metabolism
Rats, Sprague-Dawley
Subjects
Details
- Language :
- English
- ISSN :
- 2042-650X
- Volume :
- 15
- Issue :
- 14
- Database :
- MEDLINE
- Journal :
- Food & function
- Publication Type :
- Academic Journal
- Accession number :
- 38899520
- Full Text :
- https://doi.org/10.1039/d4fo01087f