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Innate immune receptors are differentially expressed in mice during experimental Schistosoma mansoni early infection.
- Source :
-
Memorias do Instituto Oswaldo Cruz [Mem Inst Oswaldo Cruz] 2024 Jun 17; Vol. 119, pp. e240013. Date of Electronic Publication: 2024 Jun 17 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Background: The impact of Schistosoma mansoni infection over the immune response and the mechanisms involved in pathogenesis are not yet completely understood.<br />Objectives: This study aimed to evaluate the expression of innate immune receptors in three distinct mouse lineages (BALB/c, C57BL/6 and Swiss) during experimental S. mansoni infection with LE strain.<br />Methods: The parasite burden, intestinal tissue oogram and presence of hepatic granulomas were evaluated at 7- and 12-weeks post infection (wpi). The mRNA expression for innate Toll-like receptors, Nod-like receptors, their adaptor molecules, and cytokines were determined at 2, 7 and 12 wpi in the hepatic tissue by real-time quantitative polymerase chain reaction (qPCR).<br />Findings: Swiss mice showed 100% of survival, had lower parasite burden and intestinal eggs, while infected BALB/c and C57BL/6 presented 80% and 90% of survival, respectively, higher parasite burden and intestinal eggs. The three mouse lineages displayed distinct patterns in the expression of innate immune receptors, their adaptor molecules and cytokines, at 2 and 7 wpi.<br />Main Conclusions: Our results suggest that the pathogenesis of S. mansoni infection is related to a dynamic early activation of innate immunity receptors and cytokines important for the control of developing worms.
- Subjects :
- Animals
Mice
Schistosoma mansoni immunology
Disease Models, Animal
Female
Toll-Like Receptors immunology
Real-Time Polymerase Chain Reaction
Parasite Egg Count
Male
RNA, Messenger
Receptors, Immunologic genetics
Receptors, Immunologic immunology
Schistosomiasis mansoni immunology
Immunity, Innate immunology
Mice, Inbred BALB C
Mice, Inbred C57BL
Cytokines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1678-8060
- Volume :
- 119
- Database :
- MEDLINE
- Journal :
- Memorias do Instituto Oswaldo Cruz
- Publication Type :
- Academic Journal
- Accession number :
- 38896633
- Full Text :
- https://doi.org/10.1590/0074-02760240013