A Fragment-Based Competitive 19 F LB-NMR Platform For Hotspot-Directed Ligand Profiling.

Ligand binding hotspots are regions of protein surfaces that form particularly favourable interactions with small molecule pharmacophores. Targeting interactions with these hotspots maximises the efficiency of ligand binding. Existing methods are capable of identifying hotspots but often lack assays to quantify ligand binding and direct elaboration at these sites. Herein, we describe a fragment-based competitive ¹⁹ F Ligand Based NMR (LB-NMR) screening platform that enables routine, quantitative ligand profiling focused at ligand-binding hotspots. As a proof of concept, the method was applied to 4'-phosphopantetheine adenylyltransferase (PPAT) from Mycobacterium abscessus (Mabs). X-ray crystallographic characterisation of the hits from a 960-member fragment screen identified three ligand-binding hotspots across the PPAT active site. From the fragment hits a collection of ¹⁹ F reporter candidates were designed and synthesised. By rigorous prioritisation and use of optimisation workflows, a single ¹⁹ F reporter molecule was generated for each hotspot. Profiling the binding of a set of structurally characterised ligands by competitive ¹⁹ F LB-NMR with this suite of ¹⁹ F reporters recapitulated the binding affinity and site ID assignments made by ITC and X-ray crystallography. This quantitative mapping of ligand binding events at hotspot level resolution establishes the utility of the fragment-based competitive ¹⁹ F LB-NMR screening platform for hotspot-directed ligand profiling.
(© 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.)