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Non-Glycosylated SARS-CoV-2 Omicron BA.5 Receptor Binding Domain (RBD) with a Native-like Conformation Induces a Robust Immune Response with Potent Neutralization in a Mouse Model.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2024 Jun 05; Vol. 29 (11). Date of Electronic Publication: 2024 Jun 05. - Publication Year :
- 2024
-
Abstract
- The Omicron BA.5 variant of SARS-CoV-2 is known for its high transmissibility and its capacity to evade immunity provided by vaccine protection against the (original) Wuhan strain. In our prior research, we successfully produced the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein in an E. coli expression system. Extensive biophysical characterization indicated that, even without glycosylation, the RBD maintained native-like conformational and biophysical properties. The current study explores the immunogenicity and neutralization capacity of the E. coli -expressed Omicron BA.5 RBD using a mouse model. Administration of three doses of the RBD without any adjuvant elicited high titer antisera of up to 7.3 × 10 <superscript>5</superscript> and up to 1.6 × 10 <superscript>6</superscript> after a booster shot. Immunization with RBD notably enhanced the population of CD44 <superscript>+</superscript> CD62L <superscript>+</superscript> T cells, indicating the generation of T cell memory. The in vitro assays demonstrated the antisera's protective efficacy through significant inhibition of the interaction between SARS-CoV-2 and its human receptor, ACE2, and through potent neutralization of a pseudovirus. These findings underscore the potential of our E. coli -expressed RBD as a viable vaccine candidate against the Omicron variant of SARS-CoV-2.
- Subjects :
- Animals
Mice
Humans
Antibodies, Viral immunology
Disease Models, Animal
Protein Domains
Glycosylation
Protein Binding
Female
Escherichia coli metabolism
T-Lymphocytes immunology
SARS-CoV-2 immunology
Spike Glycoprotein, Coronavirus immunology
Spike Glycoprotein, Coronavirus chemistry
Spike Glycoprotein, Coronavirus metabolism
Antibodies, Neutralizing immunology
COVID-19 prevention & control
COVID-19 immunology
Angiotensin-Converting Enzyme 2 metabolism
Angiotensin-Converting Enzyme 2 immunology
Angiotensin-Converting Enzyme 2 chemistry
COVID-19 Vaccines immunology
COVID-19 Vaccines chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 29
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 38893549
- Full Text :
- https://doi.org/10.3390/molecules29112676