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Lycopene Regulates Macrophage Immune Response through the Autophagy Pathway Mediated by RIPK1.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 03; Vol. 72 (26), pp. 14747-14759. Date of Electronic Publication: 2024 Jun 18. - Publication Year :
- 2024
-
Abstract
- The effects of lycopene (LP) on macrophage immune responses were evaluated in this study. Compared with the control treatment, LP treatment significantly increased cell vitality, phagocytic activity, and chemokine production in RAW264.7 cells. Additionally, compared with the control treatment, 4 μM LP treatment significantly activated autophagy, enhanced mitochondrial membrane potential, and upregulated receptor-interacting protein kinase 1 (RIPK1), while necrostatin-1 significantly reversed these effects of LP. Furthermore, compared with that in the control group, RIPK1 was significantly upregulated in the 4 μM LP and 4 μM LP + spautin-1 groups, whereas p-mTOR levels were reduced. More importantly, compared with that in the control group, p62 was significantly downregulated, and Beclin1, LC3-II, and Atg7 were upregulated in the 4 μM LP group, while spautin-1 significantly reversed these effects of LP. These results confirm that LP activates the mTOR/Beclin1/LC3/p62 autophagy signaling pathway through RIPK1, thereby enhancing the immune response of macrophages.
- Subjects :
- Animals
Mice
RAW 264.7 Cells
TOR Serine-Threonine Kinases metabolism
TOR Serine-Threonine Kinases genetics
Beclin-1 genetics
Beclin-1 metabolism
Autophagy drug effects
Lycopene pharmacology
Macrophages drug effects
Macrophages immunology
Receptor-Interacting Protein Serine-Threonine Kinases genetics
Receptor-Interacting Protein Serine-Threonine Kinases metabolism
Signal Transduction drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 72
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38889306
- Full Text :
- https://doi.org/10.1021/acs.jafc.4c02531