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Hybrid Micro-/Nanoprotein Platform Provides Endocrine-like and Extracellular Matrix-like Cell Delivery of Growth Factors.
- Source :
-
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2024 Jul 03; Vol. 16 (26), pp. 32930-32944. Date of Electronic Publication: 2024 Jun 18. - Publication Year :
- 2024
-
Abstract
- Protein materials are versatile tools in diverse biomedical fields. Among them, artificial secretory granules (SGs), mimicking those from the endocrine system, act as mechanically stable reservoirs for the sustained release of proteins as oligomeric functional nanoparticles. Only validated in oncology, the physicochemical properties of SGs, along with their combined drug-releasing and scaffolding abilities, make them suitable as smart topographies in regenerative medicine for the prolonged delivery of growth factors (GFs). Thus, considering the need for novel, safe, and cost-effective materials to present GFs, in this study, we aimed to biofabricate a protein platform combining both endocrine-like and extracellular matrix fibronectin-derived (ECM-FN) systems. This approach is based on the sustained delivery of a nanostructured histidine-tagged version of human fibroblast growth factor 2. The GF is presented onto polymeric surfaces, interacting with FN to spontaneously generate nanonetworks that absorb and present the GF in the solid state, to modulate mesenchymal stromal cell (MSC) behavior. The results show that SGs-based topographies trigger high rates of MSCs proliferation while preventing differentiation. While this could be useful in cell therapy manufacture demanding large numbers of unspecialized MSCs, it fully validates the hybrid platform as a convenient setup for the design of biologically active hybrid surfaces and in tissue engineering for the controlled manipulation of mammalian cell growth.
- Subjects :
- Humans
Fibroblast Growth Factor 2 chemistry
Fibroblast Growth Factor 2 pharmacology
Cell Proliferation drug effects
Cell Differentiation drug effects
Nanostructures chemistry
Mesenchymal Stem Cells cytology
Mesenchymal Stem Cells metabolism
Mesenchymal Stem Cells drug effects
Extracellular Matrix chemistry
Extracellular Matrix metabolism
Fibronectins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1944-8252
- Volume :
- 16
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- ACS applied materials & interfaces
- Publication Type :
- Academic Journal
- Accession number :
- 38888932
- Full Text :
- https://doi.org/10.1021/acsami.4c01210