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Investigation into Potential Allergenicity and Digestion-Resistant Linear Epitopes of Fish Skin Gelatin in Cell-Cultured Meat Scaffolds.
- Source :
-
Journal of agricultural and food chemistry [J Agric Food Chem] 2024 Jul 03; Vol. 72 (26), pp. 14922-14940. Date of Electronic Publication: 2024 Jun 17. - Publication Year :
- 2024
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Abstract
- As a key component of cell-cultured fish, fish skin gelatin (FSG)-based cell scaffold provides support structures for cell growth, proliferation, and differentiation. However, there are potential allergenicity risks contained in FSG-based scaffolds. In this study, 3D edible scaffolds were prepared by phase separation method and showed a contact angle of less than 90°, which indicated that the scaffolds were favorable for cell adhesion. Besides, the swelling ratio was greater than 200%, implying a great potential to support cell growth. The sequence homology analysis indicated that FSG was prone to cross-reaction with collagen analogues. Additionally, a food allergic model was constructed and represented that mice gavaged with cod FSG exhibited higher levels of specific antibodies, mast cell degranulation, vascular permeability, and intestinal barrier impairment than those gavaged with pangasius and tilapias FSG. Its higher allergenicity might be attributed to a higher number of digestion-resistant linear epitopes. Moreover, the higher hydrolysis degree linked to the exposure of linear epitopes to promote the combination with IgE, which was also responsible for maintaining the higher allergenicity of cod FSG. This study clarifies allergenic risks in cell-cultured fish and further study will focus on the allergenicity reduction of FSG-based cell scaffolds.
- Subjects :
- Animals
Mice
Humans
Immunoglobulin E immunology
Fishes immunology
Mice, Inbred BALB C
Mast Cells immunology
Meat analysis
Gadiformes immunology
In Vitro Meat
Gelatin chemistry
Gelatin immunology
Epitopes immunology
Epitopes chemistry
Food Hypersensitivity immunology
Allergens immunology
Allergens chemistry
Tissue Scaffolds chemistry
Skin immunology
Fish Proteins immunology
Fish Proteins chemistry
Digestion
Subjects
Details
- Language :
- English
- ISSN :
- 1520-5118
- Volume :
- 72
- Issue :
- 26
- Database :
- MEDLINE
- Journal :
- Journal of agricultural and food chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 38885638
- Full Text :
- https://doi.org/10.1021/acs.jafc.4c02336