Back to Search
Start Over
Wolbachia mediates crosstalk between miRNA and Toll pathways to enhance resistance to dengue virus in Aedes aegypti.
- Source :
-
PLoS pathogens [PLoS Pathog] 2024 Jun 17; Vol. 20 (6), pp. e1012296. Date of Electronic Publication: 2024 Jun 17 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- The obligate endosymbiont Wolbachia induces pathogen interference in the primary disease vector Aedes aegypti, facilitating the utilization of Wolbachia-based mosquito control for arbovirus prevention, particularly against dengue virus (DENV). However, the mechanisms underlying Wolbachia-mediated virus blockade have not been fully elucidated. Here, we report that Wolbachia activates the host cytoplasmic miRNA biogenesis pathway to suppress DENV infection. Through the suppression of the long noncoding RNA aae-lnc-2268 by Wolbachia wAlbB, aae-miR-34-3p, a miRNA upregulated by the Wolbachia strains wAlbB and wMelPop, promoted the expression of the antiviral effector defensin and cecropin genes through the Toll pathway regulator MyD88. Notably, anti-DENV resistance induced by Wolbachia can be further enhanced, with the potential to achieve complete virus blockade by increasing the expression of aae-miR-34-3p in Ae. aegypti. Furthermore, the downregulation of aae-miR-34-3p compromised Wolbachia-mediated virus blockade. These findings reveal a novel mechanism by which Wolbachia establishes crosstalk between the cytoplasmic miRNA pathway and the Toll pathway via aae-miR-34-3p to strengthen antiviral immune responses against DENV. Our results will aid in the advancement of Wolbachia for arbovirus control by enhancing its virus-blocking efficiency.<br />Competing Interests: Z.X. is affiliated with Guangzhou Wolbaki Biotech Co., Ltd. The other authors have declared that no competing interests exist.<br /> (Copyright: © 2024 She et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Subjects :
- Animals
Toll-Like Receptors metabolism
Toll-Like Receptors immunology
Mosquito Vectors virology
Mosquito Vectors microbiology
Mosquito Vectors immunology
Signal Transduction
RNA, Long Noncoding genetics
RNA, Long Noncoding immunology
Immunity, Innate
Symbiosis
Wolbachia physiology
Aedes microbiology
Aedes virology
Aedes immunology
MicroRNAs genetics
MicroRNAs metabolism
Dengue Virus immunology
Dengue immunology
Dengue virology
Subjects
Details
- Language :
- English
- ISSN :
- 1553-7374
- Volume :
- 20
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- PLoS pathogens
- Publication Type :
- Academic Journal
- Accession number :
- 38885278
- Full Text :
- https://doi.org/10.1371/journal.ppat.1012296