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Overexpression of arginase gene CAR1 renders yeast Saccharomyces cerevisiae acetic acid tolerance.

Authors :
Xiong L
Wang YT
Zhou MH
Takagi H
Qin J
Zhao XQ
Source :
Synthetic and systems biotechnology [Synth Syst Biotechnol] 2024 May 29; Vol. 9 (4), pp. 723-732. Date of Electronic Publication: 2024 May 29 (Print Publication: 2024).
Publication Year :
2024

Abstract

Acetic acid is a common inhibitor present in lignocellulose hydrolysate, which inhibits the ethanol production by yeast strains. Therefore, the cellulosic ethanol industry requires yeast strains that can tolerate acetic acid stress. Here we demonstrate that overexpressing a yeast native arginase-encoding gene, CAR1 , renders Saccharomyces cerevisiae acetic acid tolerance. Specifically, ethanol yield increased by 27.3% in the CAR1 -overexpressing strain compared to the control strain under 5.0 g/L acetic acid stress. The global intracellular amino acid level and compositions were further analyzed, and we found that CAR1 overexpression reduced the total amino acid content in response to acetic acid stress. Moreover, the CAR1 overexpressing strain showed increased ATP level and improved cell membrane integrity. Notably, we demonstrated that the effect of CAR1 overexpression was independent of the spermidine and proline metabolism, which indicates novel mechanisms for enhancing yeast stress tolerance. Our studies also suggest that CAR1 is a novel genetic element to be used in synthetic biology of yeast for efficient production of fuel ethanol.<br />Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.<br /> (© 2024 The Authors.)

Details

Language :
English
ISSN :
2405-805X
Volume :
9
Issue :
4
Database :
MEDLINE
Journal :
Synthetic and systems biotechnology
Publication Type :
Academic Journal
Accession number :
38882181
Full Text :
https://doi.org/10.1016/j.synbio.2024.05.013