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An "off-on-enhanced on" electrochemiluminescence biosensor based on resonance energy transfer and surface plasmon coupled 3D DNA walker for ultra-sensitive detection of microRNA-21.

Authors :
Li ML
Zhong MY
Zhang J
Zhang YJ
Zhang YQ
Liu Y
Li XK
Gan ST
Meng GR
Mi L
Hu YH
Zhang F
Zhang XX
Wang YZ
Source :
Analytica chimica acta [Anal Chim Acta] 2024 Aug 01; Vol. 1315, pp. 342822. Date of Electronic Publication: 2024 Jun 04.
Publication Year :
2024

Abstract

In this study, a novel electrochemiluminescence (ECL) biosensor was developed to detect microRNA-21 (miRNA-21) with high sensitivity by leveraging the combined mechanisms of resonance energy transfer (RET) and surface plasmon coupling (SPC). Initially, the glassy carbon electrode (GCE) were coated with Cu-Zn-In-S quantum dots (CZIS QDs), known for their defect-related emission suitable for ECL sensing. Subsequently, a hairpin DNA H3 with gold nanoparticles (Au NPs) attached at the end was modified over the surface of the quantum dots. The Au NPs could effectively quench the ECL signals of CZIS QDs via RET. Further, a significant amount of report DNA was generated through the action of a 3D DNA walker. When the report DNA opened H3-Au NPs, the hairpin structure experienced a conformational change to a linear shape, increasing the gap between the CZIS QDs and the Au NPs. Consequently, the localized surface plasmon resonance ECL (LSPR-ECL) effect replaced ECL resonance energy transfer (ECL-RET). Moreover, the report DNA was released following the addition of H4-Au NPs, resulting in the formation of Au dimers and a surface plasma-coupled ECL (SPC-ECL) effect that enhanced the ECL intensity to 6.97-fold. The integration of new ECL-RET and SPC-ECL biosensor accurately quantified miRNA-21 concentrations from 10 <superscript>-8</superscript>  M to 10 <superscript>-16</superscript>  M with a limit of detection (LOD) of 0.08 fM, as well as successfully applied to validate human serum samples.<br />Competing Interests: Declaration of competing interest The authors declare no competing financial interest.<br /> (Copyright © 2024 Elsevier B.V. All rights reserved.)

Details

Language :
English
ISSN :
1873-4324
Volume :
1315
Database :
MEDLINE
Journal :
Analytica chimica acta
Publication Type :
Academic Journal
Accession number :
38879216
Full Text :
https://doi.org/10.1016/j.aca.2024.342822