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Monitoring and quantifying replication fork dynamics with high-throughput methods.

Monitoring and quantifying replication fork dynamics with high-throughput methods.

Authors :
Fajri N
Petryk N
Source :
Communications biology [Commun Biol] 2024 Jun 14; Vol. 7 (1), pp. 729. Date of Electronic Publication: 2024 Jun 14.
Publication Year :
2024

Abstract

Before each cell division, eukaryotic cells must replicate their chromosomes to ensure the accurate transmission of genetic information. Chromosome replication involves more than just DNA duplication; it also includes chromatin assembly, inheritance of epigenetic marks, and faithful resumption of all genomic functions after replication. Recent progress in quantitative technologies has revolutionized our understanding of the complexity and dynamics of DNA replication forks at both molecular and genomic scales. Here, we highlight the pivotal role of these novel methods in uncovering the principles and mechanisms of chromosome replication. These technologies have illuminated the regulation of genome replication programs, quantified the impact of DNA replication on genomic mutations and evolutionary processes, and elucidated the mechanisms of replication-coupled chromatin assembly and epigenome maintenance.<br /> (© 2024. The Author(s).)

Details

Language :
English
ISSN :
2399-3642
Volume :
7
Issue :
1
Database :
MEDLINE
Journal :
Communications biology
Publication Type :
Academic Journal
Accession number :
38877080
Full Text :
https://doi.org/10.1038/s42003-024-06412-1