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The lncRNA1-miR6288b-3p-PpTCP4-PpD2 module regulates peach branch number by affecting brassinosteroid biosynthesis.
- Source :
-
The New phytologist [New Phytol] 2024 Aug; Vol. 243 (3), pp. 1050-1064. Date of Electronic Publication: 2024 Jun 14. - Publication Year :
- 2024
-
Abstract
- Branch number is one of the most important agronomic traits of fruit trees such as peach. Little is known about how LncRNA and/or miRNA modules regulate branching through transcription factors. Here, we used molecular and genetic tools to clarify the molecular mechanisms underlying brassinosteroid (BR) altering plant branching. We found that the number of sylleptic branch and BR content in pillar peach ('Zhaoshouhong') was lower than those of standard type ('Okubo'), and exogenous BR application could significantly promote branching. PpTCP4 expressed great differentially comparing 'Zhaoshouhong' with 'Okubo'. PpTCP4 could directly bind to DWARF2 (PpD2) and inhibited its expression. PpD2 was the only one differentially expressed key gene in the path of BR biosynthesis. At the same time, PpTCP4 was identified as a target of miR6288b-3p. LncRNA1 could act as the endogenous target mimic of miR6288b-3p and repress expression of miR6288b-3p. Three deletions and five SNP sites of lncRNA1 promoter were found in 'Zhaoshouhong', which was an important cause of different mRNA level of PpTCP4 and BR content. Moreover, overexpressed PpTCP4 significantly inhibited branching. A novel mechanism in which the lncRNA1-miR6288b-3p-PpTCP4-PpD2 module regulates peach branching number was proposed.<br /> (© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
- Subjects :
- Promoter Regions, Genetic genetics
Base Sequence
Polymorphism, Single Nucleotide genetics
Genes, Plant
RNA, Long Noncoding genetics
RNA, Long Noncoding metabolism
MicroRNAs genetics
MicroRNAs metabolism
Prunus persica genetics
Prunus persica growth & development
Prunus persica metabolism
Gene Expression Regulation, Plant
Brassinosteroids metabolism
Brassinosteroids biosynthesis
Plant Proteins genetics
Plant Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1469-8137
- Volume :
- 243
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The New phytologist
- Publication Type :
- Academic Journal
- Accession number :
- 38872462
- Full Text :
- https://doi.org/10.1111/nph.19903