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Baicalein attenuates oxidative damage in mice haematopoietic cells through regulation of PDGFRβ.
- Source :
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Molecular and cellular probes [Mol Cell Probes] 2024 Aug; Vol. 76, pp. 101966. Date of Electronic Publication: 2024 Jun 15. - Publication Year :
- 2024
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Abstract
- Platelet-derived growth factor receptor β (PDGFRβ) plays a crucial role in murine haematopoiesis. Baicalein (BAI), a naturally occurring flavonoid, can alleviate disease damage through anti-oxidative, anti-apoptotic, and anti-inflammatory mechanisms. However, whether BAI attenuates oxidative damage in murine haematopoietic cells by PDGFRβ remains unexplored. In this study, we utilized a tert-butyl hydroperoxide (TBHP)-induced BaF3 cell injury model and an ionising radiation (IR)-induced mice injury model to investigate the impact of the presence or absence of PDGFRβ on the pharmacological effects of BAI. In addition, the BAI-PDGFRβ interaction was characterized by molecular docking and dynamics simulations. The results show that a specific concentration of BAI led to increased cell viability, reduced reactive oxygen species (ROS) content, upregulated nuclear factor erythroid 2-related factor 2 (NRF2) expression, and its downstream target genes heme oxygenase 1 (HO-1) and NAD(P)H Quinone Dehydrogenase 1 (NQO1), and activated protein kinase B (AKT) pathway in cells expressing PDGFRβ plasmid and experiencing damage. Similarly, BAI elevated lineage <superscript>-</superscript> Sca1 <superscript>+</superscript> cKIT <superscript>+</superscript> (LSK) cell proportion, promoted haematopoietic restoration, enhanced NRF2-mediated antioxidant response in PDGFRβ <superscript>+/+</superscript> mice. However, despite BAI usage, PDGFRβ knockout mice (PDGFRβ <superscript>-/-</superscript> ) showed lower LSK proportion and less antioxidant capacity than the total body irradiation (TBI) group. Furthermore, we demonstrated an interaction between BAI and PDGFRβ at the molecular level. Collectively, our results indicate that BAI attenuates oxidative stress injury and helps promote haematopoietic cell recovery through regulation of PDGFRβ.<br />Competing Interests: Declaration of competing interest The authors declare no competing interests.<br /> (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
- Subjects :
- Animals
Mice
Hematopoietic Stem Cells drug effects
Hematopoietic Stem Cells metabolism
Signal Transduction drug effects
Cell Survival drug effects
Cell Line
Male
Proto-Oncogene Proteins c-akt metabolism
tert-Butylhydroperoxide pharmacology
Molecular Docking Simulation
Heme Oxygenase-1 metabolism
Heme Oxygenase-1 genetics
Antioxidants pharmacology
Mice, Inbred C57BL
Receptor, Platelet-Derived Growth Factor beta metabolism
Oxidative Stress drug effects
Flavanones pharmacology
NF-E2-Related Factor 2 metabolism
NF-E2-Related Factor 2 genetics
Reactive Oxygen Species metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1096-1194
- Volume :
- 76
- Database :
- MEDLINE
- Journal :
- Molecular and cellular probes
- Publication Type :
- Academic Journal
- Accession number :
- 38866345
- Full Text :
- https://doi.org/10.1016/j.mcp.2024.101966