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Confinement promotes nematic alignment of spindle-shaped cells during Drosophila embryogenesis.
- Source :
-
Development (Cambridge, England) [Development] 2024 Jul 01; Vol. 151 (13). Date of Electronic Publication: 2024 Jun 28. - Publication Year :
- 2024
-
Abstract
- Tissue morphogenesis is often controlled by actomyosin networks pulling on adherens junctions (AJs), but junctional myosin levels vary. At an extreme, the Drosophila embryo amnioserosa forms a horseshoe-shaped strip of aligned, spindle-shaped cells lacking junctional myosin. What are the bases of amnioserosal cell interactions and alignment? Compared with surrounding tissue, we find that amnioserosal AJ continuity has lesser dependence on α-catenin, the mediator of AJ-actomyosin association, and greater dependence on Bazooka/Par-3, a junction-associated scaffold protein. Microtubule bundles also run along amnioserosal AJs and support their long-range curvilinearity. Amnioserosal confinement is apparent from partial overlap of its spindle-shaped cells, its outward bulging from surrounding tissue and from compressive stress detected within the amnioserosa. Genetic manipulations that alter amnioserosal confinement by surrounding tissue also result in amnioserosal cells losing alignment and gaining topological defects characteristic of nematically ordered systems. With Bazooka depletion, confinement by surrounding tissue appears to be relatively normal and amnioserosal cells align despite their AJ fragmentation. Overall, the fully elongated amnioserosa appears to form through tissue-autonomous generation of spindle-shaped cells that nematically align in response to confinement by surrounding tissue.<br />Competing Interests: Competing interests The authors declare no competing or financial interests.<br /> (© 2024. Published by The Company of Biologists Ltd.)
- Subjects :
- Animals
Microtubules metabolism
Drosophila melanogaster embryology
Drosophila melanogaster metabolism
Embryo, Nonmammalian metabolism
Embryo, Nonmammalian cytology
alpha Catenin metabolism
Actomyosin metabolism
Morphogenesis
Drosophila embryology
Cell Shape
Intracellular Signaling Peptides and Proteins
Drosophila Proteins metabolism
Drosophila Proteins genetics
Adherens Junctions metabolism
Embryonic Development
Subjects
Details
- Language :
- English
- ISSN :
- 1477-9129
- Volume :
- 151
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Development (Cambridge, England)
- Publication Type :
- Academic Journal
- Accession number :
- 38864272
- Full Text :
- https://doi.org/10.1242/dev.202577