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Multi-omics Analysis Reveals Key Gut Microbiota and Metabolites Closely Associated with Huntington's Disease.

Authors :
Qian SX
Bao YF
Li XY
Dong Y
Zhang XL
Wu ZY
Source :
Molecular neurobiology [Mol Neurobiol] 2025 Jan; Vol. 62 (1), pp. 351-365. Date of Electronic Publication: 2024 Jun 08.
Publication Year :
2025

Abstract

Dysbiosis of the gut microbiota is closely associated with neurodegenerative diseases, including Huntington's disease (HD). Gut microbiome-derived metabolites are key factors in host-microbiome interactions. This study aimed to investigate the crucial gut microbiome and metabolites in HD and their correlations. Fecal and serum samples from 11 to 26 patients with HD, respectively, and 16 and 23 healthy controls, respectively, were collected. The fecal samples were used for shotgun metagenomics while the serum samples for metabolomics analysis. Integrated analysis of the metagenomics and metabolomics data was also conducted. Firmicutes, Bacteroidota, Proteobacteria, Uroviricota, Actinobacteria, and Verrucomicrobia were the dominant phyla. At the genus level, the presence of Bacteroides, Faecalibacterium, Parabacteroides, Alistipes, Dialister, and Christensenella was higher in HD patients, while the abundance of Lachnospira, Roseburia, Clostridium, Ruminococcus, Blautia, Butyricicoccus, Agathobaculum, Phocaeicola, Coprococcus, and Fusicatenibacter decreased. A total of 244 differential metabolites were identified and found to be enriched in the glycerophospholipid, nucleotide, biotin, galactose, and alpha-linolenic acid metabolic pathways. The AUC value from the integrated analysis (1) was higher than that from the analysis of the gut microbiota (0.8632). No significant differences were found in the ACE, Simpson, Shannon, Sobs, and Chao indexes between HD patients and controls. Our study determined crucial functional gut microbiota and potential biomarkers associated with HD pathogenesis, providing new insights into the role of the gut microbiota-brain axis in HD occurrence and development.<br />Competing Interests: Declarations. Ethics Approval and Consent to Participate: This study was approved by the Ethics Committees of the Second Affiliated Hospital of Zhejiang University (approval no. 2015-048) and the Second Affiliated Hospital of Jiaxing University (approval no. 2022SW064-02). Written informed consents were obtained from all participants. Consent for Publication: Not applicable. Competing Interests: The authors declare no competing interests.<br /> (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Details

Language :
English
ISSN :
1559-1182
Volume :
62
Issue :
1
Database :
MEDLINE
Journal :
Molecular neurobiology
Publication Type :
Academic Journal
Accession number :
38850348
Full Text :
https://doi.org/10.1007/s12035-024-04271-9