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Limited usefulness of the IS 6110 touchdown-PCR in blood for tuberculin skin test false-negative cattle with serological response to Mycobacterium bovis .
- Source :
-
Frontiers in veterinary science [Front Vet Sci] 2024 May 20; Vol. 11, pp. 1359205. Date of Electronic Publication: 2024 May 20 (Print Publication: 2024). - Publication Year :
- 2024
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Abstract
- Ante-mortem diagnosis of bovine tuberculosis (bTB) is based mainly on the tuberculin skin test (TST) and the ɣ-IFN release assay (IGRA). Some infected animals escape screening tests, thus, limit herd sanitation. Previous reports have suggested a predominant pattern of multi-organ lesions attributable to Mycobacterium bovis (the causative agent of bTB) bacteraemia. A case-control study was conducted to investigate blood PCR as an alternative tool for improving ante-mortem detection of TST false-negative bovines. Cases comprised 70 TST false-negative bovines (cases), which were serology positive, and controls included 81 TST positive bovines; all of them confirmed as infected with M. bovis . Detection of the IS 6110 target through touchdown blood-PCR (IS 6110 TD-PCR) was performed. The positivity of the blood-PCR was 27.2% in the control group. This performance was similar to the 15% obtained among cases ( p = 0.134). Most cases identified by the IS 6110 TD-PCR exhibited focalized lesions ( p = 0.002). Results demonstrated that blood-PCR could detect TST false-negative cattle, even if they are negative for IGRA. Considering that cases exhibited humoral response to M. bovis , further studies conducted in a pre-serological stage could provide evidence about the real contribution of the technique in herds.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2024 Encinas, Ferrara Muñiz, Sammarruco, Ruiz Menna, Garro, Delgado, Macías, Magnano, Zumárraga, Garbaccio and Eirin.)
Details
- Language :
- English
- ISSN :
- 2297-1769
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- Frontiers in veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 38835898
- Full Text :
- https://doi.org/10.3389/fvets.2024.1359205