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Expression Patterns of Grainyhead-Like 2 and Ovo-Like 2 in Mouse Mammary Gland Development During Pregnancy, Lactation, and Weaning.
- Source :
-
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2024 Jul 04; Vol. 30 (3), pp. 552-563. - Publication Year :
- 2024
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Abstract
- Grainyhead-like 2 (Grhl2) is a transcription factor that regulates cell adhesion genes in mammary ductal development and serves as a repressor of the epithelial-mesenchymal transition. Conversely, Ovo-like2 (Ovol2) is a target gene of Grhl2 but functions as a substitute in Grhl2-deficient mice, facilitating successful epithelial barrier formation and lumen expansion in kidney-collecting ductal epithelial cells. Our objective was to examine the expression patterns of Grhl2, Ovol2, and their associated genes during the intricate phases of mouse mammary gland development. The mRNA expression of Grhl2 and Ovol2 increased after pregnancy. We observed Grhl2 protein presence in the epithelial cell's region, coinciding with acini formation, and its signal significantly correlated with E-cadherin (Cdh1) expression. However, Ovol2 was present in the epithelial region without a correlation with Cdh1. Similarly, Zeb1, a mesenchymal transcription factor, showed Cdh1-independent expression. Subsequently, we explored the interaction between Rab25, a small G protein, and Grhl2/Ovol2. The expressions of Grhl2 and Ovol2 exhibited a strong correlation with Rab25 and claudin-4, a tight junction protein. These findings suggest that Grhl2 and Ovol2 may collaborate to regulate genes associated with cell adhesion and are crucial for maintaining epithelial integrity during the different phases of mammary gland development.<br />Competing Interests: Conflict of Interest All authors declare that they have no competing interest.<br /> (© The Author(s) 2024. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our siteāfor further information please contact journals.permissions@oup.com.)
- Subjects :
- Animals
Female
Mice
Pregnancy
DNA-Binding Proteins genetics
DNA-Binding Proteins metabolism
Epithelial Cells metabolism
Claudin-4 genetics
Claudin-4 metabolism
Cadherins
Transcription Factors genetics
Transcription Factors metabolism
Mammary Glands, Animal growth & development
Mammary Glands, Animal metabolism
Lactation genetics
Weaning
Subjects
Details
- Language :
- English
- ISSN :
- 1435-8115
- Volume :
- 30
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
- Publication Type :
- Academic Journal
- Accession number :
- 38833344
- Full Text :
- https://doi.org/10.1093/mam/ozae049