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Immunoprotective efficacy evaluation of OmpTS subunit vaccine against Aeromonas hydrophila infection in Megalobrama amblycephala.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2024 Aug; Vol. 151, pp. 109665. Date of Electronic Publication: 2024 Jun 01. - Publication Year :
- 2024
-
Abstract
- Bacterial septicemia in freshwater fish is mainly caused by Aeromonas hydrophila infection, which affects the development of aquaculture industry. In the context of sustainable aquaculture, subunit vaccines are of great values because they play positive roles in reducing the overuse of antibiotics and protecting aquatic animals against bacterial infection. In this study, the recombinant outer membrane protein OmpTS of A. hydrophila were used as subunit vaccine to immunize Megalobrama amblycephala, and its immunoprotective effect and host immune responses were evaluated. The survival rates of the vaccinated groups after bacterial infection were significantly higher than that of the control group, especially of the OmpTS high-dose vaccinated group. The better protective effects of vaccinated groups might be attributed to the increased levels of serum IgM-specific antibody titer, the reduced relative abundance of A. hydrophila in various tissues, the increased number of immune-positive cells with different epitopes, the up-regulated expression levels of immune-related genes, and the enhanced activities of antibacterial enzymes. In conclusion, OmpTS subunit vaccine could strongly induce host immune responses in M. amblycephala, thereby enhancing both cellular and humoral immunity, which exhibited excellent and effective immunoprotective efficacy.<br /> (Copyright © 2024 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacterial Outer Membrane Proteins immunology
Immunity, Humoral
Aeromonas hydrophila immunology
Fish Diseases prevention & control
Fish Diseases immunology
Gram-Negative Bacterial Infections veterinary
Gram-Negative Bacterial Infections immunology
Gram-Negative Bacterial Infections prevention & control
Bacterial Vaccines immunology
Bacterial Vaccines administration & dosage
Vaccines, Subunit immunology
Vaccines, Subunit administration & dosage
Cyprinidae immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 151
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 38830521
- Full Text :
- https://doi.org/10.1016/j.fsi.2024.109665