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Evaluation of two commercial diagnostic methods for HHV-8 viral load assessment.
- Source :
-
IJID regions [IJID Reg] 2024 May 06; Vol. 11, pp. 100374. Date of Electronic Publication: 2024 May 06 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Objectives: Human herpesvirus-8 (HHV-8) can cause Kaposi's sarcoma or B lymphoproliferative disorders such as multicentric Castleman disease. Patient follow-up is based on assessing the HHV-8 viral load, which is usually achieved using real-time polymerase chain reaction (PCR). The HHV-8 Premix r-gene kit (BioMérieux) was used by some laboratories in the past, but BioMérieux ceased the production and distribution of this kit in 2021-2022. Other kits need to be tested so that they can be used for diagnostic purposes. Here we evaluated two commercial kits: HHV8 ELITe MGB Kit (ELITech) and Quanty HHV-8 (Clonit) and compared them with the HHV-8 Premix r-gene kit.<br />Methods: We used whole blood samples that had previously been tested with the HHV-8 Premix r-gene kit for diagnostic purposes. Overall, 46 samples (37 HHV-8-positive and 9 HHV-8-negative) were tested with the ELITe MGB Kit and 37 (29 HHV-8-positive and 8 HHV-8-negative) with the Quanty HHV-8 kit. The different methods were compared using Bland-Altman and Passing-Bablok tests with Analyse-it software.<br />Results: Qualitative results were concordant except for one HHV-8 low-positive sample that was found to be negative by the ELITe MGB Kit. The quantitative results were also concordant; both kits showed mean differences of 0.58 log <subscript>10</subscript> copies/ml and 0.73 log <subscript>10</subscript> copies/ml, respectively, compared to the Premix r-gene kit.<br />Conclusions: Both the methods tested produced acceptable results and could be used for diagnostic purposes. It should be remembered that there is no international standard for HHV-8 quantification and that patients should always be followed using the same method.<br />Competing Interests: The authors have no competing interests to declare.<br /> (© 2024 The Authors.)
Details
- Language :
- English
- ISSN :
- 2772-7076
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- IJID regions
- Publication Type :
- Academic Journal
- Accession number :
- 38827633
- Full Text :
- https://doi.org/10.1016/j.ijregi.2024.100374