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Contribution of FOS in neutrophils to venous thromboembolism via miR-144 based on bioinformatic prediction and validation.
- Source :
-
Journal of cellular and molecular medicine [J Cell Mol Med] 2024 Jun; Vol. 28 (11), pp. e18370. - Publication Year :
- 2024
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Abstract
- The Finkel-Biskis-Jinkins Osteosarcoma (c-Fos; encoded by FOS) plays an important role in several cardiovascular diseases, including atherosclerosis and stroke. However, the relationship between FOS and venous thromboembolism (VTE) remains unknown. We identified differentially expressed genes in Gene Expression Omnibus dataset, GSE48000, comprising VTE patients and healthy individuals, and analysed them using CIBERSORT and weighted co-expression network analysis (WGCNA). FOS and CD46 expressions were significantly downregulated (FOS p = 2.26E-05, CD64 p = 8.83E-05) and strongly linked to neutrophil activity in VTE. We used GSE19151 and performed PCR to confirm that FOS and CD46 had diagnostic potential for VTE; however, only FOS showed differential expression by PCR and ELISA in whole blood samples. Moreover, we found that hsa-miR-144 which regulates FOS expression was significantly upregulated in VTE. Furthermore, FOS expression was significantly downregulated in neutrophils of VTE patients (p = 0.03). RNA sequencing performed on whole blood samples of VTE patients showed that FOS exerted its effects in VTE via the leptin-mediated adipokine signalling pathway. Our results suggest that FOS and related genes or proteins can outperform traditional clinical markers and may be used as diagnostic biomarkers for VTE.<br /> (© 2024 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
- Subjects :
- Female
Humans
Male
Biomarkers blood
Biomarkers metabolism
Gene Expression Profiling
Gene Expression Regulation
Gene Regulatory Networks
Computational Biology
MicroRNAs genetics
MicroRNAs blood
MicroRNAs metabolism
Neutrophils metabolism
Proto-Oncogene Proteins c-fos genetics
Proto-Oncogene Proteins c-fos metabolism
Venous Thromboembolism blood
Venous Thromboembolism genetics
Venous Thromboembolism metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1582-4934
- Volume :
- 28
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of cellular and molecular medicine
- Publication Type :
- Academic Journal
- Accession number :
- 38818568
- Full Text :
- https://doi.org/10.1111/jcmm.18370