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Integrated analysis of colorectal cancer metastasis identifies characteristics of tumor cell during metastasis.
- Source :
-
Gastroenterology report [Gastroenterol Rep (Oxf)] 2024 May 30; Vol. 12, pp. goae055. Date of Electronic Publication: 2024 May 30 (Print Publication: 2024). - Publication Year :
- 2024
-
Abstract
- Background: Metastasis is the main cause of death in colorectal cancer (CRC). Metastasis is a sequential and dynamic process, but the development of tumor cells during this process is unclear. In this study, we aimed to reveal characteristics of tumor cell subset during CRC metastasis.<br />Methods: Single-cell RNA sequence CRC data of normal epithelium, non-metastatic primary tumor, metastatic primary tumor, and liver metastases from gene expression omnibus (GEO) dataset were analyzed to reveal characteristics of CRC metastasis. Primary tumor tissues of three non-metastatic CRC and three metastatic CRC patients from Union Hospital of Tongji Medical College (Wuhan, China) were used to verify the characteristics of CRC metastasis.<br />Results: We identified a metastasis-related cancer cell subset EP1, which was characterized with a high expression of KRT17 , LAMC2 , EMP1 , and PLAC8 . EP1 had an enhanced cell-cell interaction, which interacted with SPP <superscript>+</superscript> macrophages and drove them toward anti-inflammatory and immunosuppressive phenotype. Dynamic changes in genes and TF regulons during the metastasis were also revealed.<br />Conclusions: This study advanced our understanding of the development of tumor cells during CRC metastasis and further identified metastasis-related subset and potential therapeutic targets for the treatment and prevention of CRC metastasis.<br />Competing Interests: The authors declare that there is no conflict of interest in this study.<br /> (© The Author(s) 2024. Published by Oxford University Press and Sixth Affiliated Hospital of Sun Yat-sen University.)
Details
- Language :
- English
- ISSN :
- 2052-0034
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Gastroenterology report
- Publication Type :
- Academic Journal
- Accession number :
- 38818308
- Full Text :
- https://doi.org/10.1093/gastro/goae055