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Synergy between Laminin-Derived Elastin-like Polypeptides (LELPs) Optimizes Cell Spreading.
- Source :
-
Biomacromolecules [Biomacromolecules] 2024 Jul 08; Vol. 25 (7), pp. 4001-4013. Date of Electronic Publication: 2024 May 30. - Publication Year :
- 2024
-
Abstract
- A major component of the extracellular matrix (ECM), laminins, modulates cells via diverse receptors. Their fragments have emerging utility as components of "ECM-mimetics" optimized to promote cell-based therapies. Recently, we reported that a bioactive laminin peptide known as A99 enhanced cell binding and spreading via fusion to an elastin-like polypeptide (ELP). The ELP "handle" serves as a rapid, noncovalent strategy to concentrate bioactive peptide mixtures onto a surface. We now report that this strategy can be further generalized across an expanded panel of additional laminin-derived elastin-like polypeptides (LELPs). A99 (AGTFALRGDNPQG), A2G80 (VQLRNGFPYFSY), AG73 (RKRLQVQLSIRT), and EF1m (LQLQEGRLHFMFD) all promote cell spreading while showing morphologically distinct F-actin formation. Equimolar mixtures of A99:A2G80-LELPs have synergistic effects on adhesion and spreading. Finally, three of these ECM-mimetics promote the neurite outgrowth of PC-12 cells. The evidence presented here demonstrates the potential of ELPs to deposit ECM-mimetics with applications in regenerative medicine, cell therapy, and tissue engineering.
Details
- Language :
- English
- ISSN :
- 1526-4602
- Volume :
- 25
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Biomacromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 38814168
- Full Text :
- https://doi.org/10.1021/acs.biomac.4c00144